TY - JOUR
T1 - Concordance in anti-OmpC and anti-I2 indicate the influence of genetic predisposition, results of a European study of twins with Crohn's disease
AU - Amcoff, K.
AU - Joossens, M.
AU - Pierik, M.J.
AU - Jonkers, D.
AU - Bohr, J.
AU - Joossens, S.
AU - Romberg-Camps, M.
AU - Nyhlin, N.
AU - Wickbom, A.
AU - Rutgeerts, P.J.
AU - Tysk, C.
AU - Bodin, L.
AU - Colombel, J.F.
AU - Vermeire, S.
AU - Halfvarson, J.
PY - 2016/6
Y1 - 2016/6
N2 - An adaptive immunological response to microbial antigens has been observed in Crohn's disease (CD). Intriguingly, this serological response precedes the diagnosis in some patients and has also been observed in healthy relatives. We aimed to determine whether genetic factors are implicated in this response in a CD twin cohort.In total, 82 twin pairs (Leuven n = 13, Maastricht n = 8, A-rebro n = 61) took part: 81 pairs with CD (concordant monozygotic n = 16, discordant monozygotic n = 22, concordant dizygotic n = 3, discordant dizygotic n = 40) and 1 monozygotic pair with both CD and ulcerative colitis. Serology for Pseudomonas fluorescens-related protein (anti-I2), Escherichia coli outer membrane porin C (anti-OmpC), CBir1flagellin (anti-CBir1) and antibodies to oligomannan (anti-Saccharomyces cerevisiae antibody [ASCA]) was determined by standardized enzyme-linked immunoassay.All markers were more often present in CD twins than in their healthy twin siblings. Using the intraclass correlation coefficient (ICC), agreements in concentrations of anti-OmpC and anti-I2 were observed in discordant monozygotic but not in discordant dizygotic twin pairs with CD (anti-OmpC, ICC 0.80 and -0.02, respectively) and (anti-I2, ICC 0.56 and 0.05, respectively). In contrast, no agreements were found in anti-CBir, immunoglobulin (Ig) G ASCA and ASCA IgA.We show that anti-I2 and anti-CBir1 statuses have specificity for CD and confirm previous reported specificities for anti-OmpC and ASCA. Based on quantitative analyses and observed ICCs, genetics seems to predispose to the anti-OmpC and anti-I2 response but less to ASCA and anti-CBir1 responses.
AB - An adaptive immunological response to microbial antigens has been observed in Crohn's disease (CD). Intriguingly, this serological response precedes the diagnosis in some patients and has also been observed in healthy relatives. We aimed to determine whether genetic factors are implicated in this response in a CD twin cohort.In total, 82 twin pairs (Leuven n = 13, Maastricht n = 8, A-rebro n = 61) took part: 81 pairs with CD (concordant monozygotic n = 16, discordant monozygotic n = 22, concordant dizygotic n = 3, discordant dizygotic n = 40) and 1 monozygotic pair with both CD and ulcerative colitis. Serology for Pseudomonas fluorescens-related protein (anti-I2), Escherichia coli outer membrane porin C (anti-OmpC), CBir1flagellin (anti-CBir1) and antibodies to oligomannan (anti-Saccharomyces cerevisiae antibody [ASCA]) was determined by standardized enzyme-linked immunoassay.All markers were more often present in CD twins than in their healthy twin siblings. Using the intraclass correlation coefficient (ICC), agreements in concentrations of anti-OmpC and anti-I2 were observed in discordant monozygotic but not in discordant dizygotic twin pairs with CD (anti-OmpC, ICC 0.80 and -0.02, respectively) and (anti-I2, ICC 0.56 and 0.05, respectively). In contrast, no agreements were found in anti-CBir, immunoglobulin (Ig) G ASCA and ASCA IgA.We show that anti-I2 and anti-CBir1 statuses have specificity for CD and confirm previous reported specificities for anti-OmpC and ASCA. Based on quantitative analyses and observed ICCs, genetics seems to predispose to the anti-OmpC and anti-I2 response but less to ASCA and anti-CBir1 responses.
KW - Crohn's disease
KW - serology
KW - genetics
KW - INFLAMMATORY-BOWEL-DISEASE
KW - SACCHAROMYCES-CEREVISIAE ANTIBODIES
KW - MICROBIAL ANTIGENS
KW - CLINICAL CHARACTERISTICS
KW - UNAFFECTED RELATIVES
KW - FAMILIAL EXPRESSION
KW - MANNAN ANTIBODIES
KW - SERUM ANTIBODIES
KW - DIAGNOSIS
KW - FLAGELLIN
U2 - 10.1093/ecco-jcc/jjw021
DO - 10.1093/ecco-jcc/jjw021
M3 - Article
C2 - 26818662
SN - 1873-9946
VL - 10
SP - 695
EP - 702
JO - Journal of Crohn's & Colitis
JF - Journal of Crohn's & Colitis
IS - 6
ER -