BACKGROUND: Intestinal microbiome may play a role in the pathogenesis of coeliac disease (CD). Studies comparing intestinal microbiome in children with and without CD are contradictory. AIM: To compare the composition and diversity of the duodenal mucosa-associated microbiome in children with untreated CD and control children without CD and to identify specific gut bacteria associated with CD at diagnosis. METHODS: Total microbiome profile in small bowel biopsies of 42 children (21 with untreated CD and 21 age-matched controls) were analyzed by means of IS-pro, a 16S-23S interspacer (IS) region-based profiling method. RESULTS: Both groups showed a similar mucosa-associated microbiome pattern and diversity, with high concentrations of the genera Streptococcus, Lactobacillus, and Clostridium. CONCLUSION: Mucosa-associated duodenal microbiome composition and diversity did not differ between children with untreated CD and control children. Duodenal mucosa-associated bacteria do not seem to play an important role in the pathogenesis of CD.
- coeliac disease
- duodenal mucosa-associated microbiome
- INTESTINAL MICROBIOTA
de Meij, T. G., Budding, A. E., Grasman, M. E., Kneepkens, C. M., Savelkoul, P. H., & Mearin, M. L. (2013). Composition and diversity of the duodenal mucosa-associated microbiome in children with untreated coeliac disease. Scandinavian Journal of Gastroenterology, 48(5), 530-536. https://doi.org/10.3109/00365521.2013.775666