Composition and diversity of the duodenal mucosa-associated microbiome in children with untreated coeliac disease

T.G. de Meij, A.E. Budding, M.E. Grasman, C.M. Kneepkens, P.H. Savelkoul, M.L. Mearin

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

BACKGROUND: Intestinal microbiome may play a role in the pathogenesis of coeliac disease (CD). Studies comparing intestinal microbiome in children with and without CD are contradictory. AIM: To compare the composition and diversity of the duodenal mucosa-associated microbiome in children with untreated CD and control children without CD and to identify specific gut bacteria associated with CD at diagnosis. METHODS: Total microbiome profile in small bowel biopsies of 42 children (21 with untreated CD and 21 age-matched controls) were analyzed by means of IS-pro, a 16S-23S interspacer (IS) region-based profiling method. RESULTS: Both groups showed a similar mucosa-associated microbiome pattern and diversity, with high concentrations of the genera Streptococcus, Lactobacillus, and Clostridium. CONCLUSION: Mucosa-associated duodenal microbiome composition and diversity did not differ between children with untreated CD and control children. Duodenal mucosa-associated bacteria do not seem to play an important role in the pathogenesis of CD.
Original languageEnglish
Pages (from-to)530-536
Number of pages7
JournalScandinavian Journal of Gastroenterology
Volume48
Issue number5
DOIs
Publication statusPublished - May 2013

Keywords

  • coeliac disease
  • duodenal mucosa-associated microbiome
  • IS-pro
  • pathogenesis
  • INTESTINAL MICROBIOTA
  • CHILDHOOD
  • BACTERIA
  • IDENTIFICATION
  • MICROFLORA
  • EXPRESSION
  • DIAGNOSIS

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