Abstract
BACKGROUND: Intestinal microbiome may play a role in the pathogenesis of coeliac disease (CD). Studies comparing intestinal microbiome in children with and without CD are contradictory. AIM: To compare the composition and diversity of the duodenal mucosa-associated microbiome in children with untreated CD and control children without CD and to identify specific gut bacteria associated with CD at diagnosis. METHODS: Total microbiome profile in small bowel biopsies of 42 children (21 with untreated CD and 21 age-matched controls) were analyzed by means of IS-pro, a 16S-23S interspacer (IS) region-based profiling method. RESULTS: Both groups showed a similar mucosa-associated microbiome pattern and diversity, with high concentrations of the genera Streptococcus, Lactobacillus, and Clostridium. CONCLUSION: Mucosa-associated duodenal microbiome composition and diversity did not differ between children with untreated CD and control children. Duodenal mucosa-associated bacteria do not seem to play an important role in the pathogenesis of CD.
Original language | English |
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Pages (from-to) | 530-536 |
Number of pages | 7 |
Journal | Scandinavian Journal of Gastroenterology |
Volume | 48 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2013 |
Keywords
- coeliac disease
- duodenal mucosa-associated microbiome
- IS-pro
- pathogenesis
- INTESTINAL MICROBIOTA
- CHILDHOOD
- BACTERIA
- IDENTIFICATION
- MICROFLORA
- EXPRESSION
- DIAGNOSIS