Comparison of Intestinal Microcirculation and Wound Healing in a Rat Model

Jochen Grommes*, Marcel Binneboesel, Christian D. Klink, Klaus T. von Trotha, Karina Schleimer, Michael J. Jacobs, Ulf P. Neumann, Carsten J. Krones

*Corresponding author for this work

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9 Citations (Web of Science)

Abstract

If a colonic or small bowel lesion is not accessible for endoscopic mucosal resection, enterotomy is a possible although rarely performed surgical technique. It has never been compared to circular anastomosis regarding leakage rate, bowel wall perfusion, and wound healing. Thus, the aim of this basic experimental scientific study was to investigate perianastomotic microcirculation and wound healing.Forty rats were divided into four groups (1 jejunal anastomosis, 2 jejunal enterotomy, 3 colonic anastomosis, and 4 colonic enterotomy). Following anastomosis and enterotomy, the intestinal perfusion was measured using laser fluorescence angiography (IC-View). On postoperative day 7, the surface of the mucosal villi, expression of matrix metalloproteinases (MMP) 2, 8, 9, and 13, and the number of proliferating cells (Ki67) as well as the collagen types I/III ratio were analyzed.The perianastomotic microperfusion was significantly reduced in all groups compared to the reference region. The perianastomotic perfusion index was significantly reduced in group 1 compared with group 2, whereas the perfusion index in group 3 was slightly but not significantly reduced in comparison to group 4. Ki67 was elevated in both circular anastomosis groups. Surface of the mucosal villi, MMP expression, and collagen type I/III ratio revealed no significant differences.Our study affirms the theoretical consideration of a better microperfusion of the bowel wall following an antimesenterial enterotomy and demonstrates that enterotomy is not inferior compared to circular anastomosis. Even though enterotomy is a rarely used surgical technique, it should be regarded as a possible alternative in particular situations.
Original languageEnglish
Pages (from-to)46-52
JournalJournal of Investigative Surgery
Volume26
Issue number1
DOIs
Publication statusPublished - Feb 2013

Keywords

  • anastomosis
  • enterotomy
  • MMPs
  • collagen type I/III ratio
  • intestinal wound healing
  • bowel wall perfusion

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