Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions

Thijs Vandenryt*, Andrea Pohl, Bart van Grinsven, Ronald Thoelen, Ward De Ceuninck, Patrick Wagner, Joerg Opitz

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Web of Science)

Abstract

In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1x PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS.

Original languageEnglish
Pages (from-to)14650-14661
Number of pages12
JournalSensors
Volume13
Issue number11
DOIs
Publication statusPublished - Nov 2013
Externally publishedYes

Keywords

  • SPR
  • EIS
  • flow-cell
  • lab on chip
  • Protein A
  • immunoglobulin
  • C-REACTIVE PROTEIN
  • OPTICAL IMMUNOSENSOR
  • LABEL-FREE
  • BIOSENSOR
  • SENSOR
  • QUANTIFICATION
  • IMMUNOASSAYS
  • PLATFORM
  • MILK
  • GOLD

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