Abstract
In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1x PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS.
Original language | English |
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Pages (from-to) | 14650-14661 |
Number of pages | 12 |
Journal | Sensors |
Volume | 13 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2013 |
Externally published | Yes |
Keywords
- SPR
- EIS
- flow-cell
- lab on chip
- Protein A
- immunoglobulin
- C-REACTIVE PROTEIN
- OPTICAL IMMUNOSENSOR
- LABEL-FREE
- BIOSENSOR
- SENSOR
- QUANTIFICATION
- IMMUNOASSAYS
- PLATFORM
- MILK
- GOLD