Abstract
Different developments necessary for the application of different mass spectrometry imaging techniques on skeletal tissue are presented in this thesis. Sample preparation protocols for undecalcified bone and fracture hematoma were optimized for analysis with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Time-dependent changes in the lipid profiles were observed during fracture healing in human fracture hematoma. In addition, different lipid profiles were seen between the citrulline supplementation and control group during different fracture healing phases in a rat model. Protein and pathway analyses showed an enhancement of fracture healing in the citrulline supplementation group in comparison to the control group. An automated setup for laser-assisted rapid evaporative ionisation mass spectrometry (LA-REIMS) was developed for the analysis of sample surfaces with height variations. This setup allowed for the visualization of molecular distributions for a wide variety of samples, including human femoral heads. The different methodological developments and the applications shown in this thesis can be used for future research in skeletal tissue and its healing processes to improve molecular understanding and outcome prediction.
Original language | English |
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Qualification | Doctor of Philosophy |
Awarding Institution |
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Supervisors/Advisors |
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Award date | 6 Mar 2023 |
Place of Publication | Maastricht |
Publisher | |
Print ISBNs | 9789464589337 |
DOIs | |
Publication status | Published - 2023 |
Keywords
- mass spectrometry imaging
- skeletal trauma
- bone healing
- methodological developments