Circulating miR-185-5p as a Potential Biomarker for Arrhythmogenic Right Ventricular Cardiomyopathy

C. Sacchetto; Z. Mohseni; R.M.W. Colpaert; L. Vitiello; M. De Bortoli; I.G.C. Vonhogen; K. Xiao; G. Poloni; A. Lorenzon; C. Romualdi; R. Bariani; E. Mazzotti; L. Daliento; B. Bauce; D. Corrado; T. Thum; A. Rampazzo; L.J. de Windt; M. Calore

doi:10.3390/cells10102578

Circulating miR-185-5p as a Potential Biomarker for Arrhythmogenic Right Ventricular Cardiomyopathy

C. Sacchetto, Z. Mohseni, R.M.W. Colpaert, L. Vitiello, M. De Bortoli, I.G.C. Vonhogen, K. Xiao, G. Poloni, A. Lorenzon, C. Romualdi, R. Bariani, E. Mazzotti, L. Daliento, B. Bauce, D. Corrado, T. Thum, A. Rampazzo, L.J. de Windt, M. Calore^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Web of Science)

Abstract

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a genetic cardiac disease characterized by progressive myocardial fibro-fatty replacement, arrhythmias and risk of sudden death. Its diagnosis is challenging and often it is achieved after disease onset or postmortem. In this study, we sought to identify circulating microRNAs (miRNAs) differentially expressed in ARVC patients compared to healthy controls. In the pilot study, we screened the expression of 754 miRNAs from 21 ARVC patients and 20 healthy controls. After filtering the miRNAs considering a log fold-change cut-off of & PLUSMN;1, p-value < 0.05, we selected five candidate miRNAs for a subsequent validation study in which we used TaqMan-based real-time PCR to analyse samples from 37 ARVC patients and 30 healthy controls. We found miR-185-5p significantly upregulated in ARVC patients. Receiver operating characteristic analysis indicated an area under the curve of 0.854, corroborating the link of this miRNA and ARVC pathophysiology.</p>

Original language	English
Article number	2578
Number of pages	9
Journal	Cells
Volume	10
Issue number	10
DOIs	https://doi.org/10.3390/cells10102578
Publication status	Published - 1 Oct 2021

Keywords

arrhythmogenic right ventricular cardiomyopathy
MicroRNAs
circulating microRNAs
heart failure
biomarkers
genetics
MICRORNA

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10.3390/cells10102578Licence: CC BY

Cite this

Sacchetto, C., Mohseni, Z., Colpaert, R. M. W., Vitiello, L., De Bortoli, M., Vonhogen, I. G. C., Xiao, K., Poloni, G., Lorenzon, A., Romualdi, C., Bariani, R., Mazzotti, E., Daliento, L., Bauce, B., Corrado, D., Thum, T., Rampazzo, A., de Windt, L. J., & Calore, M. (2021). Circulating miR-185-5p as a Potential Biomarker for Arrhythmogenic Right Ventricular Cardiomyopathy. Cells, 10(10), [2578]. https://doi.org/10.3390/cells10102578

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title = "Circulating miR-185-5p as a Potential Biomarker for Arrhythmogenic Right Ventricular Cardiomyopathy",

abstract = "Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a genetic cardiac disease characterized by progressive myocardial fibro-fatty replacement, arrhythmias and risk of sudden death. Its diagnosis is challenging and often it is achieved after disease onset or postmortem. In this study, we sought to identify circulating microRNAs (miRNAs) differentially expressed in ARVC patients compared to healthy controls. In the pilot study, we screened the expression of 754 miRNAs from 21 ARVC patients and 20 healthy controls. After filtering the miRNAs considering a log fold-change cut-off of & PLUSMN;1, p-value < 0.05, we selected five candidate miRNAs for a subsequent validation study in which we used TaqMan-based real-time PCR to analyse samples from 37 ARVC patients and 30 healthy controls. We found miR-185-5p significantly upregulated in ARVC patients. Receiver operating characteristic analysis indicated an area under the curve of 0.854, corroborating the link of this miRNA and ARVC pathophysiology.",

keywords = "arrhythmogenic right ventricular cardiomyopathy, MicroRNAs, circulating microRNAs, heart failure, biomarkers, genetics, MICRORNA",

author = "C. Sacchetto and Z. Mohseni and R.M.W. Colpaert and L. Vitiello and {De Bortoli}, M. and I.G.C. Vonhogen and K. Xiao and G. Poloni and A. Lorenzon and C. Romualdi and R. Bariani and E. Mazzotti and L. Daliento and B. Bauce and D. Corrado and T. Thum and A. Rampazzo and {de Windt}, L.J. and M. Calore",

year = "2021",

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Sacchetto, C, Mohseni, Z , Colpaert, RMW, Vitiello, L, De Bortoli, M, Vonhogen, IGC, Xiao, K, Poloni, G, Lorenzon, A, Romualdi, C, Bariani, R, Mazzotti, E, Daliento, L, Bauce, B, Corrado, D, Thum, T, Rampazzo, A, de Windt, LJ & Calore, M 2021, 'Circulating miR-185-5p as a Potential Biomarker for Arrhythmogenic Right Ventricular Cardiomyopathy', Cells, vol. 10, no. 10, 2578. https://doi.org/10.3390/cells10102578

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T1 - Circulating miR-185-5p as a Potential Biomarker for Arrhythmogenic Right Ventricular Cardiomyopathy

AU - Sacchetto, C.

AU - Mohseni, Z.

AU - Colpaert, R.M.W.

AU - Vitiello, L.

AU - De Bortoli, M.

AU - Vonhogen, I.G.C.

AU - Xiao, K.

AU - Poloni, G.

AU - Lorenzon, A.

AU - Romualdi, C.

AU - Bariani, R.

AU - Mazzotti, E.

AU - Daliento, L.

AU - Bauce, B.

AU - Corrado, D.

AU - Thum, T.

AU - Rampazzo, A.

AU - de Windt, L.J.

AU - Calore, M.

PY - 2021/10/1

Y1 - 2021/10/1

N2 - Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a genetic cardiac disease characterized by progressive myocardial fibro-fatty replacement, arrhythmias and risk of sudden death. Its diagnosis is challenging and often it is achieved after disease onset or postmortem. In this study, we sought to identify circulating microRNAs (miRNAs) differentially expressed in ARVC patients compared to healthy controls. In the pilot study, we screened the expression of 754 miRNAs from 21 ARVC patients and 20 healthy controls. After filtering the miRNAs considering a log fold-change cut-off of & PLUSMN;1, p-value < 0.05, we selected five candidate miRNAs for a subsequent validation study in which we used TaqMan-based real-time PCR to analyse samples from 37 ARVC patients and 30 healthy controls. We found miR-185-5p significantly upregulated in ARVC patients. Receiver operating characteristic analysis indicated an area under the curve of 0.854, corroborating the link of this miRNA and ARVC pathophysiology.

AB - Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a genetic cardiac disease characterized by progressive myocardial fibro-fatty replacement, arrhythmias and risk of sudden death. Its diagnosis is challenging and often it is achieved after disease onset or postmortem. In this study, we sought to identify circulating microRNAs (miRNAs) differentially expressed in ARVC patients compared to healthy controls. In the pilot study, we screened the expression of 754 miRNAs from 21 ARVC patients and 20 healthy controls. After filtering the miRNAs considering a log fold-change cut-off of & PLUSMN;1, p-value < 0.05, we selected five candidate miRNAs for a subsequent validation study in which we used TaqMan-based real-time PCR to analyse samples from 37 ARVC patients and 30 healthy controls. We found miR-185-5p significantly upregulated in ARVC patients. Receiver operating characteristic analysis indicated an area under the curve of 0.854, corroborating the link of this miRNA and ARVC pathophysiology.

KW - arrhythmogenic right ventricular cardiomyopathy

KW - MicroRNAs

KW - circulating microRNAs

KW - heart failure

KW - biomarkers

KW - genetics

KW - MICRORNA

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SN - 2073-4409

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