Circulating but not faecal short-chain fatty acids are related to insulin sensitivity, lipolysis and GLP-1 concentrations in humans

Mattea Muller, Manuel A. Gonzalez Hernandez, Gijs H. Goossens, Dorien Reijnders, Jens J. Holst, Johan W. E. Jocken, Hans van Eijk, Emanuel E. Canfora, Ellen E. Blaak*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

94 Citations (Web of Science)

Abstract

Microbial-derived short-chain fatty acids (SCFA) acetate, propionate and butyrate may provide a link between gut microbiota and whole-body insulin sensitivity (IS). In this cross-sectional study (160 participants, 64% male, BMI: 19.2-41.0kg/m(2), normal or impaired glucose metabolism), associations between SCFA (faecal and fasting circulating) and circulating metabolites, substrate oxidation and IS were investigated. In a subgroup (n = 93), IS was determined using a hyperinsulinemic-euglycemic clamp. Data were analyzed using multiple linear regression analysis adjusted for sex, age and BMI. Fasting circulating acetate, propionate and butyrate concentrations were positively associated with fasting GLP-1 concentrations. Additionally, circulating SCFA were negatively related to whole-body lipolysis (glycerol), triacylglycerols and free fatty acids levels (standardized (std) beta adjusted (adj) - 0.190, P = 0.023; std beta adj - 0.202, P = 0.010; std beta adj - 0.306, P = 0.001, respectively). Circulating acetate and propionate were, respectively, negatively and positively correlated with IS (M-value: std beta adj - 0.294, P <0.001; std beta adj 0.161, P = 0.033, respectively). We show that circulating rather than faecal SCFA were associated with GLP-1 concentrations, whole-body lipolysis and peripheral IS in humans. Therefore, circulating SCFA are more directly linked to metabolic health, which indicates the need to measure circulating SCFA in human prebiotic/probiotic intervention studies as a biomarker/mediator of effects on host metabolism.

Original languageEnglish
Article number12515
Number of pages9
JournalScientific Reports
Volume9
DOIs
Publication statusPublished - 29 Aug 2019

Keywords

  • CHROMATOGRAPHY-MASS SPECTROMETRY
  • COLONIC FERMENTATION
  • GUT MICROBIOTA
  • GLUCOSE-HOMEOSTASIS
  • SODIUM-BUTYRATE
  • ADIPOSE-TISSUE
  • DIETARY FIBER
  • BODY-WEIGHT
  • ACETATE
  • METABOLISM

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