Cigarette smoke-induced transgenerational alterations in genome stability in cord blood of human F1 offspring.

J. Laubenthal, O. Zlobinskaya, K. Poterlowicz, A. Baumgartner, M. R. Gdula, E. Fthenou, M. Keramarou, S.J. Hepworth, J.C. Kleinjans, F.J. van Schooten, G. Brunborg, R.W.L. Godschalk, T. E. Schmid, D. Anderson

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The relevance of preconceptional and prenatal toxicant exposures for genomic stability in offspring is difficult to analyze in human populations, because gestational exposures usually cannot be separated from preconceptional exposures. To analyze the roles of exposures during gestation and conception on genomic stability in the offspring, stability was assessed via the Comet assay and highly sensitive, semiautomated confocal laser scans of gammaH2AX foci in cord, maternal, and paternal blood as well as spermatozoa from 39 families in Crete, Greece, and the United Kingdom. With use of multivariate linear regression analysis with backward selection, preconceptional paternal smoking (% tail DNA: P>0.032; gammaH2AX foci: P>0.018) and gestational maternal (% tail DNA: P>0.033) smoking were found to statistically significantly predict DNA damage in the cord blood of F1 offspring. Maternal passive smoke exposure was not identified as a predictor of DNA damage in cord blood, indicating that the effect of paternal smoking may be transmitted via the spermatozoal genome. Taken together, these studies reveal a role for cigarette smoke in the induction of DNA alterations in human F1 offspring via exposures of the fetus in utero or the paternal germline. Moreover, the identification of transgenerational DNA alterations in the unexposed F1 offspring of smoking-exposed fathers supports the claim that cigarette smoke is a human germ cell mutagen.-Laubenthal, J., Zlobinskaya, O., Poterlowicz, K., Baumgartner, A., Gdula, M. R., Fthenou, E., Keramarou, M., Hepworth, S. J., Kleinjans, J. C. S., van Schooten, F.-J., Brunborg, G., Godschalk, R. W., Schmid, T. E., Anderson, D. Cigarette smoke-induced transgenerational alterations in genome stability in cord blood of human F1 offspring.
Original languageEnglish
Pages (from-to)3946-3956
Number of pages11
JournalFaseb Journal
Volume26
Issue number10
DOIs
Publication statusPublished - Oct 2012

Keywords

  • toxicants
  • DNA damage
  • male germline
  • transmission
  • germ cell mutagen
  • TOBACCO-SMOKE
  • DNA-DAMAGE
  • CRITICAL WINDOWS
  • GENE-EXPRESSION
  • EXPOSURE
  • PREGNANCY
  • MOTHERS
  • LINE
  • SUSCEPTIBILITY
  • RNA

Cite this

Laubenthal, J., Zlobinskaya, O., Poterlowicz, K., Baumgartner, A., Gdula, M. R., Fthenou, E., Keramarou, M., Hepworth, S. J., Kleinjans, J. C., van Schooten, F. J., Brunborg, G., Godschalk, R. W. L., Schmid, T. E., & Anderson, D. (2012). Cigarette smoke-induced transgenerational alterations in genome stability in cord blood of human F1 offspring. Faseb Journal, 26(10), 3946-3956. https://doi.org/10.1096/fj.11-201194