Abstract
A large fraction of the skin-homing T-cell population resides in the skin even under resting, non-inflammatory conditions. Here, we used a crawl-out culture method to retrieve T cells from human skin and characterized them using flow cytometric analysis. On average, 48000 viable, non-proliferating cells were retrieved per biopsy. We found that human skin contains a larger fraction of IL-17-, IL-4-, IL-10- and IL-22-positive T cells as compared with paired blood samples. Our research indicates that it is feasible to use the crawl-out method in combination with flow cytometry to characterize T-cell subpopulations in patient-derived skin biopsies. This method enables further study of the skin immune system and could function as a valuable tool for evaluation of the effects of immunotherapy in skin diseases.
Original language | English |
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Pages (from-to) | 554-555 |
Journal | Experimental Dermatology |
Volume | 22 |
Issue number | 8 |
DOIs | |
Publication status | Published - Aug 2013 |
Keywords
- cytokines
- human
- skin
- skin resident T cells
- T cell subsets