Characterization of skin-resident T cells using a crawl-out method and flow cytometry

Jacqueline Poot*, Marielle Thewissen, Darren Booi, Christina Nieuwhof

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Web of Science)


A large fraction of the skin-homing T-cell population resides in the skin even under resting, non-inflammatory conditions. Here, we used a crawl-out culture method to retrieve T cells from human skin and characterized them using flow cytometric analysis. On average, 48000 viable, non-proliferating cells were retrieved per biopsy. We found that human skin contains a larger fraction of IL-17-, IL-4-, IL-10- and IL-22-positive T cells as compared with paired blood samples. Our research indicates that it is feasible to use the crawl-out method in combination with flow cytometry to characterize T-cell subpopulations in patient-derived skin biopsies. This method enables further study of the skin immune system and could function as a valuable tool for evaluation of the effects of immunotherapy in skin diseases.
Original languageEnglish
Pages (from-to)554-555
JournalExperimental Dermatology
Issue number8
Publication statusPublished - Aug 2013


  • cytokines
  • human
  • skin
  • skin resident T cells
  • T cell subsets

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