Abstract
In development, differentiation from a pluripotent state results in global epigenetic changes, although the extent to which this occurs in induced pluripotent stem cell-based neuronal models has not been extensively characterized. In the present study, induced pluripotent stem cell colonies (33Qn1 line) were differentiated and collected at four time-points, with DNA methylation assessed using the Illumina Infinium Human Methylation EPIC BeadChip array. Dynamic changes in DNA methylation occurring during differentiation were investigated using a data-driven trajectory inference method. We identified a large number of Bonferroni-significant loci that showed progressive alterations in DNA methylation during neuronal differentiation. A gene-gene interaction network analysis identified 60 densely connected genes that were influential in the differentiation of neurons, with STAT3 being the gene with the highest connectivity.
Original language | English |
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Article number | 647981 |
Number of pages | 9 |
Journal | Frontiers in Cell and Developmental Biology |
Volume | 9 |
DOIs | |
Publication status | Published - 1 Jul 2021 |
Keywords
- aging
- DNA methylation
- EPIC array
- epigenetics
- epigenome-wide association study
- induced pluripotent stem cells
- neuronal differentiation
- trajectory inference
- METHYLATION