Characterization of blunt chest trauma in a long-term porcine model of severe multiple trauma

K Horst*, T P Simon, R Pfeifer, M Teuben, K Almahmoud, Q Zhi, S Aguiar Santos, C Castelar Wembers, S Leonhardt, N Heussen, P Störmann, B Auner, B Relja, I Marzi, A T Haug, M van Griensven, M Kalbitz, M Huber-Lang, R Tolba, L K ReissS Uhlig, G Marx, H C Pape, F Hildebrand

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Chest trauma has a significant relevance on outcome after severe trauma. Clinically, impaired lung function typically occurs within 72 hours after trauma. However, the underlying pathophysiological mechanisms are still not fully elucidated. Therefore, we aimed to establish an experimental long-term model to investigate physiological, morphologic and inflammatory changes, after severe trauma. Male pigs (sus scrofa) sustained severe trauma (including unilateral chest trauma, femur fracture, liver laceration and hemorrhagic shock). Additionally, non-injured animals served as sham controls. Chest trauma resulted in severe lung damage on both CT and histological analyses. Furthermore, severe inflammation with a systemic increase of IL-6 (p = 0.0305) and a local increase of IL-8 in BAL (p = 0.0009) was observed. The pO2/FiO2 ratio in trauma animals decreased over the observation period (p < 0.0001) but not in the sham group (p = 0.2967). Electrical Impedance Tomography (EIT) revealed differences between the traumatized and healthy lung (p < 0.0001). In conclusion, a clinically relevant, long-term model of blunt chest trauma with concomitant injuries has been developed. This reproducible model allows to examine local and systemic consequences of trauma and is valid for investigation of potential diagnostic or therapeutic options. In this context, EIT might represent a radiation-free method for bedside diagnostics.

Original languageEnglish
Article number39659
Pages (from-to)39659
Number of pages13
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 21 Dec 2016
Externally publishedYes

Keywords

  • Animals
  • Bronchoalveolar Lavage Fluid
  • Disease Models, Animal
  • Electric Impedance
  • Hemodynamics
  • Inflammation/pathology
  • Interleukin-6/metabolism
  • Interleukin-8/metabolism
  • Lung/physiopathology
  • Lung Injury/physiopathology
  • Male
  • Multiple Trauma/physiopathology
  • Shock, Hemorrhagic/pathology
  • Swine
  • Thoracic Injuries/diagnostic imaging
  • Tomography
  • Tomography, X-Ray Computed
  • Wounds, Nonpenetrating/diagnostic imaging
  • INJURY
  • VENTILATION-PERFUSION RELATIONSHIPS
  • ELECTRICAL-IMPEDANCE TOMOGRAPHY
  • PULMONARY CONTUSION
  • INTRAHOSPITAL TRANSPORT
  • MECHANICAL VENTILATION
  • COMPUTED-TOMOGRAPHY
  • RESPIRATORY-DISTRESS-SYNDROME
  • LUNG CONTUSION
  • INFLAMMATORY RESPONSE

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