TY - JOUR
T1 - Challenges to, and prospects for, reverse engineering the gastrointestinal tract using organoids
AU - Kakni, Panagiota
AU - Truckenmüller, Roman
AU - Habibović, Pamela
AU - Giselbrecht, Stefan
N1 - Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.
PY - 2022/8
Y1 - 2022/8
N2 - For over a decade, organoids mimicking the development, physiology, and disease of the digestive system have been a topic of broad interest and intense study. Establishing organoid models that recapitulate all distinct regions of the gastrointestinal tract (GIT) has proven challenging since each tissue surrogate requires tailor-made modifications of the original protocol to generate intestinal organoids. In this review, we discuss the challenges and current advances of the GIT organoid models. Moreover, we envision the next-generation GIT organoids as integrated organoid models, able to recapitulate structural and functional characteristics of multiple regions of the digestive tube in a single in vitro model. We discuss these new trends and provide an outlook for the future of GIT in vitro models.
AB - For over a decade, organoids mimicking the development, physiology, and disease of the digestive system have been a topic of broad interest and intense study. Establishing organoid models that recapitulate all distinct regions of the gastrointestinal tract (GIT) has proven challenging since each tissue surrogate requires tailor-made modifications of the original protocol to generate intestinal organoids. In this review, we discuss the challenges and current advances of the GIT organoid models. Moreover, we envision the next-generation GIT organoids as integrated organoid models, able to recapitulate structural and functional characteristics of multiple regions of the digestive tube in a single in vitro model. We discuss these new trends and provide an outlook for the future of GIT in vitro models.
U2 - 10.1016/j.tibtech.2022.01.006
DO - 10.1016/j.tibtech.2022.01.006
M3 - (Systematic) Review article
C2 - 35221125
SN - 0167-7799
VL - 40
SP - 932
EP - 944
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 8
ER -