TY - JOUR
T1 - ChAdOx1 vaccination, blood coagulation, and inflammation:
T2 - No effect on coagulation but increased interleukin-6
AU - Willems, L.H.
AU - Nagy, M.
AU - Ten Cate, H.
AU - Spronk, H.M.H.
AU - Jacobs, L.M.C.
AU - Kranendonk, J.
AU - van Leeuwen, M.
AU - Meijer, D.
AU - Middeldorp, S.
AU - Groh, L.A.
AU - Warle, M.C.
PY - 2021/12/1
Y1 - 2021/12/1
N2 - Background: Vaccination is the leading approach in combatting the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. ChAdOx1 nCoV-19 vaccination (ChAdOx1) has been linked to a higher frequency of rare thrombosis and thromboembolism. This study aimed to explore markers related to the blood coagulation system activation and inflammation, before and after ChAdOx1 vaccination.Patients and Methods: An observational cohort study including 40 health care workers. Whole blood samples were collected before, and either 1 or 2 days after vaccination. Activated coagulation factors in complex with their natural inhibitors were determined by custom ELISAs, including thrombin:antithrombin (T:AT), kallikrein:C1esterase-inhibitor (PKa:C1Inh), factor(F)IXa:AT, FXa:AT, FXIaAT, FXIa:alpha-1-antitrypsin (alpha 1AT), FXIa:C1inh, and FVIIa:AT. Plasma concentrations of interleukin (IL)-6 and IL-18 were quantified via ELISA. Analyses were performed using Wilcoxon signed-rank test.Results: Levels of FVIIa:AT decreased with a median (IQR) of 707 (549-1028) pg/ml versus 598 (471-996) pg/mI, p = 0.01; and levels of IL-6 increased, 4.0 (1.9-6.8) pg/ml versus 6.9 (3.6-12.2) pg/ml, p = 0.02, after vaccination. No changes were observed in T:AT, PKa:C1Inh, FIXa:AT, FXa:AT, FXIaAT, FX1a:alpha 1AT, FXIa:C1inh, and IL-18.Conclusion: ChAdOx1 leads to an inflammatory response with increased levels of IL6. We did not observe activation of the blood coagulation system 1-2 days following vaccination.
AB - Background: Vaccination is the leading approach in combatting the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. ChAdOx1 nCoV-19 vaccination (ChAdOx1) has been linked to a higher frequency of rare thrombosis and thromboembolism. This study aimed to explore markers related to the blood coagulation system activation and inflammation, before and after ChAdOx1 vaccination.Patients and Methods: An observational cohort study including 40 health care workers. Whole blood samples were collected before, and either 1 or 2 days after vaccination. Activated coagulation factors in complex with their natural inhibitors were determined by custom ELISAs, including thrombin:antithrombin (T:AT), kallikrein:C1esterase-inhibitor (PKa:C1Inh), factor(F)IXa:AT, FXa:AT, FXIaAT, FXIa:alpha-1-antitrypsin (alpha 1AT), FXIa:C1inh, and FVIIa:AT. Plasma concentrations of interleukin (IL)-6 and IL-18 were quantified via ELISA. Analyses were performed using Wilcoxon signed-rank test.Results: Levels of FVIIa:AT decreased with a median (IQR) of 707 (549-1028) pg/ml versus 598 (471-996) pg/mI, p = 0.01; and levels of IL-6 increased, 4.0 (1.9-6.8) pg/ml versus 6.9 (3.6-12.2) pg/ml, p = 0.02, after vaccination. No changes were observed in T:AT, PKa:C1Inh, FIXa:AT, FXa:AT, FXIaAT, FX1a:alpha 1AT, FXIa:C1inh, and IL-18.Conclusion: ChAdOx1 leads to an inflammatory response with increased levels of IL6. We did not observe activation of the blood coagulation system 1-2 days following vaccination.
KW - blood coagulation
KW - COVID-19
KW - COVID-19 vaccines
KW - SARS-CoV-2
KW - thrombosis
KW - vaccination
KW - IL-6 PRODUCTION
U2 - 10.1002/rth2.12630
DO - 10.1002/rth2.12630
M3 - Article
C2 - 34934894
SN - 2475-0379
VL - 5
JO - Research and practice in thrombosis and haemostasis
JF - Research and practice in thrombosis and haemostasis
IS - 8
M1 - e12630
ER -