Cell-type specific expression and regulation of apolipoprotein D and E in human endometrium

Ariane Germeyer*, Edison Capp, Franziska Schlicksupp, Julia Jauckus, Ulrike von Rango - Hilmes, Michael von Wolff, Thomas Strowitzki

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Web of Science)

Abstract

Objective: To assess the expression and regulation of antilipoprotein D (ApoD) and antilipoprotein E (ApoE) in human endometrium. Study design: Endometrial biopsies from healthy, regularly cycling women were collected during the late proliferative and mid-secretory phase. mRNA gene expression of ApoD and ApoE was determined using real-time PCR in whole tissue, in isolated stromal (ESC), epithelial (EEC) and CD45(+) leukocytes (EIC), as well as after hormonal stimulation of ESC and EEC in vitro. Protein expression was analyzed using immunohistochemistry. Results: ApoD and ApoE mRNA was expressed in all cell types examined. A rise in ApoD mRNA expression was seen in whole endometrium, ESC, and EEC in the secretory phase, as well as after hormonal stimulation of ESC and EEC in vitro. ApoE mRNA was significantly upregulated in whole endometrium of secretory phase biopsies, while its expression was not altered by progesterone in vitro. Immunohistochemistry of whole endometrial tissue localized ApoD mainly in ESC and EEC. While ApoE was localized slightly in ESC, it was particularly noted on the surface of secretory phase endothelial cells. Conclusion: We demonstrate for the first time the cell-type and cycle dependent expression of ApoD and ApoE within human endometrium, suggesting their role in endometrial modulation.
Original languageEnglish
Pages (from-to)487-491
JournalEuropean Journal of Obstetrics & Gynecology and Reproductive Biology
Volume170
Issue number2
DOIs
Publication statusPublished - Oct 2013

Keywords

  • Apolipoprotein D
  • Apolipoprotein E
  • Gene and protein expression
  • Endometrium

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