CD36 Is Important for Adipocyte Recruitment and Affects Lipolysis

Irene O. C. M. Vroegrijk, Jan Bert van Klinken, Janna A. van Diepen, Sjoerd A. A. van den Berg, Maria Febbraio, Laura K. M. Steinbusch, Jan F. C. Glatz, Louis M. Havekes, Peter J. Voshol, Patrick C. N. Rensen, Ko Willems van Dijk, Vanessa van Harmelen*

*Corresponding author for this work

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Abstract

Objective: The scavenger receptor CD36 facilitates the cellular uptake of long-chain fatty acids. As CD36-deficiency attenuates the development of high fat diet (HFD)-induced obesity, the role of CD36deficiency in preadipocyte recruitment and adipocyte function was set out to characterize. Design and Methods: Fat cell size and number were determined in gonadal, visceral, and subcutaneous adipose tissue of CD36(-/-) and WT mice after 6 weeks on HFD. Basal lipolysis and insulin-inhibited lipolysis were investigated in gonadal adipose tissue. Results: CD36(-/-) mice showed a reduction in adipocyte size in all fat pads. Gonadal adipose tissue also showed a lower total number of adipocytes because of a lower number of very small adipocytes (diameter <50 mu m). This was accompanied by an increased pool of preadipocytes, which suggests that CD36-deficiency reduces the capacity of preadipocytes to become adipocytes. Regarding lipolysis, in adipose tissue from CD36(-/-) mice, cAMP levels were increased and both basal and 8-bromo-cAMP stimulated lipolysis were higher. However, insulin-mediated inhibition of lipolysis was more potent in CD36(-/-) mice. Conclusions: These results indicate that during fat depot expansion, CD36-deficiency negatively affects preadipocyte recruitment and that in mature adipocytes, CD36-deficiency is associated with increased basal lipolysis and insulin responsiveness.
Original languageEnglish
Pages (from-to)2037-2045
JournalObesity
Volume21
Issue number10
DOIs
Publication statusPublished - Oct 2013

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