CCR5 and FPR1 Mediate Neutrophil Recruitment in Endotoxin-Induced Lung Injury

Jochen Grommes*, Maik Drechsler, Oliver Soehnlein

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

36 Citations (Web of Science)

Abstract

Recruitment of neutrophils, regarded as a key mechanism in acute lung injury (ALI), is orchestrated by cell adhesion molecules and chemokines. While the importance of cell adhesion molecules has been carefully investigated, little is known about the importance of chemokines and their receptors in the recruitment of neutrophils in models of ALI. Wild-type Ccr24(-/-), Ccr5(-/-), Fpr1(-/-) or Fpr2(-/) (-)mice were exposed to aerosolized lipopolysaccharide and the number of neutrophils in the lung tissue (intravascular, interstitial) and in the bronchoalveolar lavage was quantified. Lack of CCR5 or FPR1, but not CCR2 or FPR2, significantly reduced lung neutrophil infiltration in all compartments. Similarly, blockade of CCR5 or FPR1 with specific antagonists reduced counts of alveolar, interstitial and intravascular neutrophils. Such treatments also inhibited lung edema formation and histological lung tissue alterations, thus underscoring the protective role of CCR5 and FPR1 neutralizing strategies in ALI.
Original languageEnglish
Pages (from-to)111-116
JournalJournal of Innate Immunity
Volume6
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • Neutrophil
  • Recruitment
  • Chemokine
  • Lung injury

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