TY - JOUR
T1 - Can the composition of the intestinal microbiota predict the development of urinary tract infections?
AU - den Heijer, Casper D. J.
AU - Geerlings, Suzanne E.
AU - Prins, Jan M.
AU - Beerepoot, Marielle A. J.
AU - Stobberingh, Ellen E.
AU - Penders, John
PY - 2016/10
Y1 - 2016/10
N2 - Aim: To evaluate whether intestinal microbiota predicts the development of new-onset urinary tract infections (UTIs) in postmenopausal women with prior recurrent UTIs (rUTIs). Patients & methods: Fecal samples (n = 40) originated from women with rUTI who received 12 months' prophylaxis of either trimethoprim-sulfamethoxazole (TMP-SMX) or lactobacilli. Microbial composition was assessed by 16S rRNA pyrosequencing. Results: At baseline, fecal microbiota of women with zero and more than or equal to four UTIs during follow-up showed no significant differences. Only TMP-SMX prophylaxis resulted in reduced microbial diversity. Microbial structure of two samples from the same woman showed limited relatedness. Conclusion: In postmenopausal women with rUTI, the intestinal microbiota was not predictive for new-onset UTIs. Only TMP-SMX, and not lactobacilli, prophylaxis had effects on the microbial composition. Data in ENA: PRJEB13868.
AB - Aim: To evaluate whether intestinal microbiota predicts the development of new-onset urinary tract infections (UTIs) in postmenopausal women with prior recurrent UTIs (rUTIs). Patients & methods: Fecal samples (n = 40) originated from women with rUTI who received 12 months' prophylaxis of either trimethoprim-sulfamethoxazole (TMP-SMX) or lactobacilli. Microbial composition was assessed by 16S rRNA pyrosequencing. Results: At baseline, fecal microbiota of women with zero and more than or equal to four UTIs during follow-up showed no significant differences. Only TMP-SMX prophylaxis resulted in reduced microbial diversity. Microbial structure of two samples from the same woman showed limited relatedness. Conclusion: In postmenopausal women with rUTI, the intestinal microbiota was not predictive for new-onset UTIs. Only TMP-SMX, and not lactobacilli, prophylaxis had effects on the microbial composition. Data in ENA: PRJEB13868.
KW - 16S rRNA pyrosequencing
KW - intestinal microbiota
KW - lactobacilli
KW - postmenopausal women
KW - prophylaxis
KW - trimethoprim-sulfamethoxazole
KW - urinary tract infection
U2 - 10.2217/fmb-2016-0088
DO - 10.2217/fmb-2016-0088
M3 - Article
C2 - 27785923
SN - 1746-0913
VL - 11
SP - 1394
EP - 1404
JO - Future Microbiology
JF - Future Microbiology
IS - 11
ER -