TY - JOUR
T1 - Calpain-10 Gene and Protein Expression in Human Skeletal Muscle: Effect of Acute Lipid-Induced Insulin Resistance and Type 2 Diabetes.
AU - Norton, I.
AU - Parr, T.
AU - Chokkalingam, K.
AU - Bardsley, R.G.
AU - Ye, H.
AU - Bell, G.I.
AU - Pelsers, M.M.
AU - van Loon, L.J.
AU - Tsintzas, K.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Objective: To investigate the effect of lipid-induced insulin resistance and type 2 diabetes on skeletal muscle calpain-10 mRNA and protein levels. Research design and methods: In the first part of this study, 10 healthy subjects underwent hyperinsulinemic euglycemic (4.5 mmol/l) clamps for 6 h with intravenous infusion of either saline (CON) or a 20% Intralipid emulsion (LIPID). Skeletal muscle biopsies were taken before and after 3 and 6 h of insulin infusion and analyzed for calpain-10 mRNA and protein expression. In the second part of the study, muscle samples obtained after an overnight fast in 10 long-standing, sedentary type 2 diabetes patients, 10 sedentary, weight-matched, normoglycemic controls, and 10 age-matched, endurance trained cyclists were analyzed for calpain-10 mRNA and protein content. Results: Intralipid infusion in healthy subjects reduced whole body glucose disposal by approximately 50% (P<0.001). Calpain-10 mRNA (P=0.01) but not protein content was reduced following 6 h of insulin infusion in both the CON and LIPID trials. Skeletal muscle calpain-10 mRNA and protein content did not differ between the type 2 diabetes patients and normoglycemic controls, but there was a strong trend for total calpain-10 protein to be greater in the endurance trained athletes (P=0.06). Conclusions: These data indicate that skeletal muscle calpain-10 expression is not modified by insulin resistance per se, and suggest that hyperinsulinemia and exercise training may modulate human skeletal muscle calpain-10 expression.
AB - Objective: To investigate the effect of lipid-induced insulin resistance and type 2 diabetes on skeletal muscle calpain-10 mRNA and protein levels. Research design and methods: In the first part of this study, 10 healthy subjects underwent hyperinsulinemic euglycemic (4.5 mmol/l) clamps for 6 h with intravenous infusion of either saline (CON) or a 20% Intralipid emulsion (LIPID). Skeletal muscle biopsies were taken before and after 3 and 6 h of insulin infusion and analyzed for calpain-10 mRNA and protein expression. In the second part of the study, muscle samples obtained after an overnight fast in 10 long-standing, sedentary type 2 diabetes patients, 10 sedentary, weight-matched, normoglycemic controls, and 10 age-matched, endurance trained cyclists were analyzed for calpain-10 mRNA and protein content. Results: Intralipid infusion in healthy subjects reduced whole body glucose disposal by approximately 50% (P<0.001). Calpain-10 mRNA (P=0.01) but not protein content was reduced following 6 h of insulin infusion in both the CON and LIPID trials. Skeletal muscle calpain-10 mRNA and protein content did not differ between the type 2 diabetes patients and normoglycemic controls, but there was a strong trend for total calpain-10 protein to be greater in the endurance trained athletes (P=0.06). Conclusions: These data indicate that skeletal muscle calpain-10 expression is not modified by insulin resistance per se, and suggest that hyperinsulinemia and exercise training may modulate human skeletal muscle calpain-10 expression.
U2 - 10.1210/jc.2007-1981
DO - 10.1210/jc.2007-1981
M3 - Article
SN - 0021-972X
VL - 93
SP - 992
EP - 998
JO - Journal of Clinical Endocrinology & Metabolism
JF - Journal of Clinical Endocrinology & Metabolism
IS - 3
ER -