Calcium Phosphate Coated Electrospun Fiber Matrices as Scaffolds for Bone Tissue Engineering

Anandkumar Nandakumar, Liang Yang, Pamela Habibovic*, Clemens van Blitterswijk

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Electrospun polymeric scaffolds are used for various tissue engineering applications. In this study, we applied a biomimetic coating method to provide electrospun scaffolds from a block copolymer-poly(ethylene oxide terephthalate)-poly(buthylene terephthalate), with a calcium phosphate layer to improve their bioactivity in bone tissue engineering. The in vitro studies with human mesenchymal stem cells demonstrated cell proliferation on both uncoated and coated samples. No significant effect of calcium phosphate coating was observed on the expression of alkaline phosphatase in vitro. Implantation of scaffold-goat mesenchymal stem cells constructs subcutaneously in nude mice resulted in bone formation in the calcium phosphate coated samples, in contrast to the uncoated ones, where no new bone formation was observed. The results of this study showed that the biomimetic method can successfully be used to coat electrospun scaffolds with a calcium phosphate layer, which improved the in vivo bioactivity of the polymer.

Original languageEnglish
Pages (from-to)7380-7387
Number of pages8
JournalLangmuir
Volume26
Issue number10
DOIs
Publication statusPublished - 18 May 2010
Externally publishedYes

Keywords

  • MESENCHYMAL STEM-CELLS
  • IN-VITRO
  • OCTACALCIUM PHOSPHATE
  • BIOMIMETIC COATINGS
  • VIVO
  • TITANIUM
  • APATITE
  • GROWTH
  • MORPHOLOGY
  • RELEASE

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