Abstract
Controlling stem cell fate is the cornerstone of regenerative medicine. Cadherins have an important role in cell fate commitment and the function of cadherin-11 in the regulation of differentiation in human mesenchymal stem cells (hMSCs) has recently come to light. To better understand how cadherin-11 regulates hMSC behavior, we explored its interaction with receptor tyrosine kinases (RTK), an important family of proteins involved in a myriad of cellular functions. In this study, we provide evidence that cadherin-11, a cell adhesion protein expressed in hMSCs, regulates the activity of several RTKs, including PDGFR beta and PDGFR alpha. By knocking down cadherin-11 we found that the changes in the RTK activity caused hyperactivation of the MAPK pathways, which were sustained through the phosphorylation and nuclear translocation of ERK1/2 and subsequently caused a decrease in cell proliferation. Together these results provide compelling evidence for the important role of the interaction of cadherin-11 and RTKs in the behavior of hMSCs.
Original language | English |
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Pages (from-to) | 165-174 |
Number of pages | 10 |
Journal | Stem Cells |
Volume | 40 |
Issue number | 2 |
Early online date | 1 Jan 2022 |
DOIs | |
Publication status | Published - 16 Mar 2022 |
Keywords
- adult stem cells
- cell adhesion molecules
- cell biology
- cellular proliferation
- mesenchymal stem cells (MSCs)
- proliferation
- EPIDERMAL-GROWTH-FACTOR
- STROMAL CELLS
- RECEPTOR-BETA
- G1 PHASE
- ACTIVATION
- EXPRESSION
- BINDING