Breath condenser coatings affect measurement of biomarkers in exhaled breath condensate

P.P.R. Rosias, C.M. Robroeks, H.J. Niemarkt, A.D.M. Kester, J.H. Vernooy, J. Suykerbuyk, J. Teunissen, J. Heynens, H.J. Hendriks, Q. Jöbsis, E.D. Dompeling

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Exhaled breath condensate collection is not yet standardized. Biomarker measurements are often close to lower detection-limits. We hypothesized that adhesive properties of different condenser coatings interfere with measurements of eicosanoids and proteins in condensate.In vitro, condensate was derived from a collection-model using two test solutions (8-isoprostane, albumin) and five condenser coatings respectively (silicone, glass, aluminum, polypropylene, teflon). In vivo, condensate was collected using these five coatings and the EcoScreen((R))-condenser to measure 8-isoprostane, and three coatings (silicone, glass, EcoScreen((R))) to measure albumin.In vitro, silicone and glass coatings had significantly higher albumin recovery compared with the other coatings (p=0.03). A similar trend was observed for 8-isoprostane recovery (p=0.09). In vivo, median (interquartile range) 8-isoprostane concentrations were significantly higher using silicone (9.2 pg.ml(-1) (IQR:18.8), p<0.001) or glass (3.0 pg.ml(-1) (IQR:4.5), p<0.02) coating compared with aluminum (0.5 pg.ml(-1), IQR:2.4), polypropylene (0.5 pg.ml(-1), IQR:0.5), teflon (0.5 pg.ml(-1), IQR:0.0), and EcoScreen((R)) (0.5 pg.ml(-1), IQR:2.0). Albumin in vivo was mainly detectable using glass coating (p<0.008). In conclusion, a condenser with silicone or glass coating is more efficient for measurement of 8-isoprostane or albumin in exhaled breath condensate, than EcoScreen((R)), aluminum, polypropylene or teflon. Guidelines for exhaled breath condensate standardization should include the most valid condenser coating to measure a specific biomarker. LA - ENG PT - JOURNAL ARTICLE DEP - 20060726 TA - Eur Respir J JID - 8803460
Original languageEnglish
Pages (from-to)1036-1041
JournalEuropean Respiratory Journal
Volume28
Issue number5
DOIs
Publication statusPublished - 1 Jan 2006

Cite this