Brain-Derived Neurotrophic Factor Facilitates Functional Recovery from ALS-Cerebral Spinal Fluid-Induced Neurodegenerative Changes in the NSC-34 Motor Neuron Cell Line

Shanmukha Shruthi, R. Sumitha, Anu Mary Varghese, S. Ashok, B. K. Chandrasekhar Sagar, T. N. Sathyaprabha, A. Nalini, Boris W. Kramer, Trichur R. Raju, K. Vijayalakshmi, Phalguni Anand Alladi*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Background: The survival of motor neurons is dependent upon neurotrophic factors both during childhood and adolescence and during adult life. In disease conditions, such as in patients with amyotrophic lateral sclerosis (ALS), the mRNA levels of trophic factors like brain-derived neurotrophic factor (BDNF), insulin-like growth factor-1 (IGF-1), fibroblast growth factor-2 (FGF-2), and vascular endothelial growth factor are downregulated. This was replicated in our in vivo experimental system following the injection of cerebral spinal fluid (CSF) of sporadic ALS (ALS-CSF) patients. Objective: To evaluate the protective role of BDNF in a model of sporadic ALS patients. Methods: The expressions of endogenous BDNF, its receptor TrkB, the enzyme choline acetyl transferase (ChAT), and phosphorylated neurofilaments were studied in NSC-34 cells. The calcium-buffering and proapoptotic effects were assessed by calbindin-D28K and caspase-3 expression, respectively. Results: ALS-CSF considerably depleted the endogenous BDNF protein, while its effect on IGF-1 and FGF-2 was inconsequential; this indirectly indicates a key role for BDNF in supporting motor neuronal survival. The exogenous supplementation of BDNF reversed autocrine expression; however, it may not be completely receptor mediated, as the TrkB levels were not restored. BDNF completely revived ChAT expression. It may inhibit apoptosis by restoring Ca2+ homeostasis, since caspase- 3 and calbindin-D28K expression was back to normal. The organellar ultrastructural changes were only partially reversed. Conclusion: Our study provides evidence that BDNF supplementation ameliorates most but not all degenerative changes. The incomplete revival at the ultrastructural level signifies the requirement of factors other than BDNF for near-total protection of motor neurons, and, to an extent, it explains why only a partial success is achieved in clinical trials with BDNF in ALS patients. (C) 2016 S. Karger AG, Basel

Original languageEnglish
Pages (from-to)44-58
Number of pages15
JournalNeurodegenerative Diseases
Issue number1
Publication statusPublished - 2017


  • Brain-derived neurotrophic factor
  • Receptor tyrosine kinase B
  • Choline acetyl transferase
  • Phosphorylated neurofilaments
  • Calbindin-D28K
  • Electron microscopy
  • Immunochemistry
  • Laser scanning confocal microscopy
  • BDNF
  • CORD


Dive into the research topics of 'Brain-Derived Neurotrophic Factor Facilitates Functional Recovery from ALS-Cerebral Spinal Fluid-Induced Neurodegenerative Changes in the NSC-34 Motor Neuron Cell Line'. Together they form a unique fingerprint.

Cite this