TY - JOUR
T1 - Brain- and heart-type fatty acid-binding proteins in the brain: tissue distribution and clinical utility.
AU - Pelsers, M.M.A.L.
AU - Hanhoff, T.
AU - van der Voort, D.
AU - Arts, B.
AU - Peters, M.
AU - Ponds, R.W.H.M.
AU - Honig, A.
AU - Rudzinski, W.
AU - Spener, F.
AU - de Kruijk, J.R.
AU - Twijnstra, A.
AU - Hermens, W.T.
AU - Menheere, P.P.C.A.
AU - Glatz, J.F.
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Background: Detection of brain injury by serum markers is not a standard procedure in clinical practice, although several proteins, such as S100B, neuron-specific enolase (NSE), myelin basic protein, and glial fibrillary acidic protein, show promising results. We investigated the tissue distribution of brain- and heart-type fatty acid-binding proteins (B-FABP and H-FABP) in segments of the human brain and the potential of either protein to serve as plasma marker for diagnosis of brain injury. Methods: B-FABP and H-FABP were measured immunochemically in autopsy samples of the brain (n = 6) and in serum samples from (a) patients with mild traumatic brain injury (MTBI; n = 130) and (b) depressed patients undergoing bilateral electroconvulsive therapy (ECT; n = 14). The protein markers S100B and NSE were measured for comparison. Reference values of B-FABP and H-FABP were established in healthy individuals (n = 92). Results: The frontal, temporal, and occipital lobes, the striatum, the pons, and the cerebellum had different tissue concentrations of B-FABP and of H-FABP. B-FABP ranged from 0.8 mug/g wet weight in striatum tissue to 3.1 mug/g in frontal lobe. H-FABP was markedly higher, ranging from 16.2 mug/g wet weight in cerebellum tissue to 39.5 mug/g in pons. No B-FABP was detected in serum from healthy donors. H-FABP serum reference value was 6 mug/L. In the MTBI study, serum B-FABP was increased in 68% and H-FABP in 70% of patients compared with S100B (increased in 45%) and NSE (increased in 51% of patients). In ECT, serum B-FABP was increased in 6% of all samples (2 of 14 patients), whereas H-FABP was above its upper reference limit (6 mug/L) in 17% of all samples (8 of 14 patients), and S100B was above its upper reference limit (0.3 mug/L) in 0.4% of all samples. Conclusions: B-FABP and H-FABP patterns differ among brain tissues, with the highest concentrations in the frontal lobe and pons, respectively. However, in each part of the brain, the H-FABP concentration was at least 10 times higher than that of B-FABP. Patient studies indicate that B-FABP and H-FABP are more sensitive markers for minor brain injury than the currently used markers S100B and NSE.
AB - Background: Detection of brain injury by serum markers is not a standard procedure in clinical practice, although several proteins, such as S100B, neuron-specific enolase (NSE), myelin basic protein, and glial fibrillary acidic protein, show promising results. We investigated the tissue distribution of brain- and heart-type fatty acid-binding proteins (B-FABP and H-FABP) in segments of the human brain and the potential of either protein to serve as plasma marker for diagnosis of brain injury. Methods: B-FABP and H-FABP were measured immunochemically in autopsy samples of the brain (n = 6) and in serum samples from (a) patients with mild traumatic brain injury (MTBI; n = 130) and (b) depressed patients undergoing bilateral electroconvulsive therapy (ECT; n = 14). The protein markers S100B and NSE were measured for comparison. Reference values of B-FABP and H-FABP were established in healthy individuals (n = 92). Results: The frontal, temporal, and occipital lobes, the striatum, the pons, and the cerebellum had different tissue concentrations of B-FABP and of H-FABP. B-FABP ranged from 0.8 mug/g wet weight in striatum tissue to 3.1 mug/g in frontal lobe. H-FABP was markedly higher, ranging from 16.2 mug/g wet weight in cerebellum tissue to 39.5 mug/g in pons. No B-FABP was detected in serum from healthy donors. H-FABP serum reference value was 6 mug/L. In the MTBI study, serum B-FABP was increased in 68% and H-FABP in 70% of patients compared with S100B (increased in 45%) and NSE (increased in 51% of patients). In ECT, serum B-FABP was increased in 6% of all samples (2 of 14 patients), whereas H-FABP was above its upper reference limit (6 mug/L) in 17% of all samples (8 of 14 patients), and S100B was above its upper reference limit (0.3 mug/L) in 0.4% of all samples. Conclusions: B-FABP and H-FABP patterns differ among brain tissues, with the highest concentrations in the frontal lobe and pons, respectively. However, in each part of the brain, the H-FABP concentration was at least 10 times higher than that of B-FABP. Patient studies indicate that B-FABP and H-FABP are more sensitive markers for minor brain injury than the currently used markers S100B and NSE.
U2 - 10.1373/clinchem.2003.030361
DO - 10.1373/clinchem.2003.030361
M3 - Article
C2 - 15217991
SN - 0009-9147
VL - 50
SP - 1568
EP - 1575
JO - Clinical Chemistry
JF - Clinical Chemistry
ER -