Bone regeneration in rabbit calvarial critical-sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture

Mohammad Kamal*, Lars Andersson, Adel Al-Asfour, Alexander K. Bartella, Felix Gremse, Stefanie Rosenhain, Severino Gabato, Frank Hoelzle, Peter Kessler, Bernd Lethaus

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


The purpose of this study was to evaluate bone healing in calvarial defects using two bone graft substitute materials; biphasic beta-tricalcium phosphate/hydroxyapatite in hydrogel (ss-TCP/HA) versus composite non-demineralized xenogenic dentin with ss-TCP/HA mixture. Full thickness critical-sized defects were created bilaterally in 10 New Zealand male rabbits. Seven defects were left empty, six filled with biphasic tricalcium phosphate putty, and seven were filled with composite non-demineralized xenogenic dentin with biphasic tricalcium phosphate. Animals were sacrificed at eight weeks postoperatively and the healing of the biomaterial-filled defects was compared radiographically and by histomorphometry. Micro-computed tomography (CT) was utilized to analyze the osteogenesis and healing patterns of the defects. Quantitative analysis of volume fraction (%) of the newly formed bone and remaining graft material (FV=filling volume/TV=tissue volume) and mean intensity [HU] in the defects were evaluated. Defects filled with composite dentin with biphasic tri-calcium phosphate showed volume fraction (FV/TV) in the order of 55.81% +/- 17.72%, whereas defects filled with only biphasic tricalcium phosphate showed a fraction of 39.84% +/- 16.06%, which represent the ratio of remaining graft material and new bone formation to the tissue volume. The empty negative control defects showed a volume fraction of 19.14% +/- 8.787%. Histological analysis showed significant percentage increase in bone formation and residual graft with the composite Dentin/ss-TCP group after 8 weeks. The findings suggest that composite xenogenic dentin with biphasic tricalcium phosphate showed improved osteogenesis when compared to biphasic tricalcium phosphate without the addition of non-demineralized dentin. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 773-782, 2019.

Original languageEnglish
Pages (from-to)773-782
Number of pages10
JournalJournal of Biomedical Materials Research Part B-applied Biomaterials
Issue number3
Publication statusPublished - Apr 2019


  • bone regeneration
  • bone substitutes
  • bone transplantation
  • skull
  • X-ray micro-computed tomography
  • dentin

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