Bone regeneration in rabbit calvarial critical-sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture

Mohammad Kamal; Lars Andersson; Adel Al-Asfour; Alexander K. Bartella; Felix Gremse; Stefanie Rosenhain; Severino Gabato; Frank Hoelzle; Peter Kessler; Bernd Lethaus

doi:10.1002/jbm.b.34171

Bone regeneration in rabbit calvarial critical-sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture

Mohammad Kamal^*, Lars Andersson, Adel Al-Asfour, Alexander K. Bartella, Felix Gremse, Stefanie Rosenhain, Severino Gabato, Frank Hoelzle, Peter Kessler, Bernd Lethaus

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The purpose of this study was to evaluate bone healing in calvarial defects using two bone graft substitute materials; biphasic beta-tricalcium phosphate/hydroxyapatite in hydrogel (ss-TCP/HA) versus composite non-demineralized xenogenic dentin with ss-TCP/HA mixture. Full thickness critical-sized defects were created bilaterally in 10 New Zealand male rabbits. Seven defects were left empty, six filled with biphasic tricalcium phosphate putty, and seven were filled with composite non-demineralized xenogenic dentin with biphasic tricalcium phosphate. Animals were sacrificed at eight weeks postoperatively and the healing of the biomaterial-filled defects was compared radiographically and by histomorphometry. Micro-computed tomography (CT) was utilized to analyze the osteogenesis and healing patterns of the defects. Quantitative analysis of volume fraction (%) of the newly formed bone and remaining graft material (FV=filling volume/TV=tissue volume) and mean intensity [HU] in the defects were evaluated. Defects filled with composite dentin with biphasic tri-calcium phosphate showed volume fraction (FV/TV) in the order of 55.81% +/- 17.72%, whereas defects filled with only biphasic tricalcium phosphate showed a fraction of 39.84% +/- 16.06%, which represent the ratio of remaining graft material and new bone formation to the tissue volume. The empty negative control defects showed a volume fraction of 19.14% +/- 8.787%. Histological analysis showed significant percentage increase in bone formation and residual graft with the composite Dentin/ss-TCP group after 8 weeks. The findings suggest that composite xenogenic dentin with biphasic tricalcium phosphate showed improved osteogenesis when compared to biphasic tricalcium phosphate without the addition of non-demineralized dentin. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 773-782, 2019.

Original language	English
Pages (from-to)	773-782
Number of pages	10
Journal	Journal of Biomedical Materials Research Part B-applied Biomaterials
Volume	107
Issue number	3
DOIs	https://doi.org/10.1002/jbm.b.34171
Publication status	Published - Apr 2019

Keywords

bone regeneration
bone substitutes
bone transplantation
skull
X-ray micro-computed tomography
dentin
CALCIUM-PHOSPHATE
EXPERIMENTAL-MODEL
TISSUE-REACTIONS
AUTOGENOUS BONE
GROWTH-FACTORS
FOLLOW-UP
GRAFTS
IMPLANTS
BOVINE
REPLANTATION

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10.1002/jbm.b.34171

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Kamal, M., Andersson, L., Al-Asfour, A., Bartella, A. K., Gremse, F., Rosenhain, S., Gabato, S., Hoelzle, F., Kessler, P., & Lethaus, B. (2019). Bone regeneration in rabbit calvarial critical-sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture. Journal of Biomedical Materials Research Part B-applied Biomaterials, 107(3), 773-782. https://doi.org/10.1002/jbm.b.34171

@article{4470de0619b14765b1dad95b64082037,

title = "Bone regeneration in rabbit calvarial critical-sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture",

abstract = "The purpose of this study was to evaluate bone healing in calvarial defects using two bone graft substitute materials; biphasic beta-tricalcium phosphate/hydroxyapatite in hydrogel (ss-TCP/HA) versus composite non-demineralized xenogenic dentin with ss-TCP/HA mixture. Full thickness critical-sized defects were created bilaterally in 10 New Zealand male rabbits. Seven defects were left empty, six filled with biphasic tricalcium phosphate putty, and seven were filled with composite non-demineralized xenogenic dentin with biphasic tricalcium phosphate. Animals were sacrificed at eight weeks postoperatively and the healing of the biomaterial-filled defects was compared radiographically and by histomorphometry. Micro-computed tomography (CT) was utilized to analyze the osteogenesis and healing patterns of the defects. Quantitative analysis of volume fraction (%) of the newly formed bone and remaining graft material (FV=filling volume/TV=tissue volume) and mean intensity [HU] in the defects were evaluated. Defects filled with composite dentin with biphasic tri-calcium phosphate showed volume fraction (FV/TV) in the order of 55.81% +/- 17.72%, whereas defects filled with only biphasic tricalcium phosphate showed a fraction of 39.84% +/- 16.06%, which represent the ratio of remaining graft material and new bone formation to the tissue volume. The empty negative control defects showed a volume fraction of 19.14% +/- 8.787%. Histological analysis showed significant percentage increase in bone formation and residual graft with the composite Dentin/ss-TCP group after 8 weeks. The findings suggest that composite xenogenic dentin with biphasic tricalcium phosphate showed improved osteogenesis when compared to biphasic tricalcium phosphate without the addition of non-demineralized dentin. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 773-782, 2019.",

keywords = "bone regeneration, bone substitutes, bone transplantation, skull, X-ray micro-computed tomography, dentin, CALCIUM-PHOSPHATE, EXPERIMENTAL-MODEL, TISSUE-REACTIONS, AUTOGENOUS BONE, GROWTH-FACTORS, FOLLOW-UP, GRAFTS, IMPLANTS, BOVINE, REPLANTATION",

author = "Mohammad Kamal and Lars Andersson and Adel Al-Asfour and Bartella, {Alexander K.} and Felix Gremse and Stefanie Rosenhain and Severino Gabato and Frank Hoelzle and Peter Kessler and Bernd Lethaus",

note = "Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",

year = "2019",

month = apr,

doi = "10.1002/jbm.b.34171",

language = "English",

volume = "107",

pages = "773--782",

journal = "Journal of Biomedical Materials Research Part B-applied Biomaterials",

issn = "1552-4973",

publisher = "Wiley",

number = "3",

}

Kamal, M, Andersson, L, Al-Asfour, A, Bartella, AK, Gremse, F, Rosenhain, S, Gabato, S, Hoelzle, F, Kessler, P & Lethaus, B 2019, 'Bone regeneration in rabbit calvarial critical-sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture', Journal of Biomedical Materials Research Part B-applied Biomaterials, vol. 107, no. 3, pp. 773-782. https://doi.org/10.1002/jbm.b.34171

Bone regeneration in rabbit calvarial critical-sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture. / Kamal, Mohammad; Andersson, Lars; Al-Asfour, Adel et al.
In: Journal of Biomedical Materials Research Part B-applied Biomaterials, Vol. 107, No. 3, 04.2019, p. 773-782.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Bone regeneration in rabbit calvarial critical-sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture

AU - Kamal, Mohammad

AU - Andersson, Lars

AU - Al-Asfour, Adel

AU - Bartella, Alexander K.

AU - Gremse, Felix

AU - Rosenhain, Stefanie

AU - Gabato, Severino

AU - Hoelzle, Frank

AU - Kessler, Peter

AU - Lethaus, Bernd

PY - 2019/4

Y1 - 2019/4

N2 - The purpose of this study was to evaluate bone healing in calvarial defects using two bone graft substitute materials; biphasic beta-tricalcium phosphate/hydroxyapatite in hydrogel (ss-TCP/HA) versus composite non-demineralized xenogenic dentin with ss-TCP/HA mixture. Full thickness critical-sized defects were created bilaterally in 10 New Zealand male rabbits. Seven defects were left empty, six filled with biphasic tricalcium phosphate putty, and seven were filled with composite non-demineralized xenogenic dentin with biphasic tricalcium phosphate. Animals were sacrificed at eight weeks postoperatively and the healing of the biomaterial-filled defects was compared radiographically and by histomorphometry. Micro-computed tomography (CT) was utilized to analyze the osteogenesis and healing patterns of the defects. Quantitative analysis of volume fraction (%) of the newly formed bone and remaining graft material (FV=filling volume/TV=tissue volume) and mean intensity [HU] in the defects were evaluated. Defects filled with composite dentin with biphasic tri-calcium phosphate showed volume fraction (FV/TV) in the order of 55.81% +/- 17.72%, whereas defects filled with only biphasic tricalcium phosphate showed a fraction of 39.84% +/- 16.06%, which represent the ratio of remaining graft material and new bone formation to the tissue volume. The empty negative control defects showed a volume fraction of 19.14% +/- 8.787%. Histological analysis showed significant percentage increase in bone formation and residual graft with the composite Dentin/ss-TCP group after 8 weeks. The findings suggest that composite xenogenic dentin with biphasic tricalcium phosphate showed improved osteogenesis when compared to biphasic tricalcium phosphate without the addition of non-demineralized dentin. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 773-782, 2019.

AB - The purpose of this study was to evaluate bone healing in calvarial defects using two bone graft substitute materials; biphasic beta-tricalcium phosphate/hydroxyapatite in hydrogel (ss-TCP/HA) versus composite non-demineralized xenogenic dentin with ss-TCP/HA mixture. Full thickness critical-sized defects were created bilaterally in 10 New Zealand male rabbits. Seven defects were left empty, six filled with biphasic tricalcium phosphate putty, and seven were filled with composite non-demineralized xenogenic dentin with biphasic tricalcium phosphate. Animals were sacrificed at eight weeks postoperatively and the healing of the biomaterial-filled defects was compared radiographically and by histomorphometry. Micro-computed tomography (CT) was utilized to analyze the osteogenesis and healing patterns of the defects. Quantitative analysis of volume fraction (%) of the newly formed bone and remaining graft material (FV=filling volume/TV=tissue volume) and mean intensity [HU] in the defects were evaluated. Defects filled with composite dentin with biphasic tri-calcium phosphate showed volume fraction (FV/TV) in the order of 55.81% +/- 17.72%, whereas defects filled with only biphasic tricalcium phosphate showed a fraction of 39.84% +/- 16.06%, which represent the ratio of remaining graft material and new bone formation to the tissue volume. The empty negative control defects showed a volume fraction of 19.14% +/- 8.787%. Histological analysis showed significant percentage increase in bone formation and residual graft with the composite Dentin/ss-TCP group after 8 weeks. The findings suggest that composite xenogenic dentin with biphasic tricalcium phosphate showed improved osteogenesis when compared to biphasic tricalcium phosphate without the addition of non-demineralized dentin. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 773-782, 2019.

KW - bone regeneration

KW - bone substitutes

KW - bone transplantation

KW - skull

KW - X-ray micro-computed tomography

KW - dentin

KW - CALCIUM-PHOSPHATE

KW - EXPERIMENTAL-MODEL

KW - TISSUE-REACTIONS

KW - AUTOGENOUS BONE

KW - GROWTH-FACTORS

KW - FOLLOW-UP

KW - GRAFTS

KW - IMPLANTS

KW - BOVINE

KW - REPLANTATION

U2 - 10.1002/jbm.b.34171

DO - 10.1002/jbm.b.34171

M3 - Article

C2 - 30253039

SN - 1552-4973

VL - 107

SP - 773

EP - 782

JO - Journal of Biomedical Materials Research Part B-applied Biomaterials

JF - Journal of Biomedical Materials Research Part B-applied Biomaterials

IS - 3

ER -

Bone regeneration in rabbit calvarial critical-sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture

Abstract

Keywords

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