BACKGROUND: Picky or fussy eating is common in early childhood and associated with a decreased preference for a variety of foods. The aim of the current study was to experimentally test which sensory food feature is associated with food acceptance, which, in turn is an indication for fussy eating, in young children (between 32 and 48 months). In a repeated-measures-design, three sensory features were manipulated separately (i.e., colour, texture and taste) while keeping the other two features constant. The baseline measurement consisted of a well-liked yoghurt, which was presented before each manipulation variant. The number of spoons that children (N = 32) consumed from each variant were registered as behavioural indication for food acceptance. Another aim was to evaluate how the behavioural measurement of food acceptance would be related to parental reports of their child's fussy eating behaviour and parental feeding styles. In addition, this study explored if children's body-mass index (BMI) was related to the behavioural measurement of food acceptance and parental reports of fussy eating behaviour. RESULTS: The manipulation of food texture caused a significant decrease in intake. Colour and taste manipulations of the yoghurt did not affect children's intake. Parental reports of children's fussy eating behaviour and parental feeding styles were not related to the behavioural observation of food acceptance. The behavioural measurement of food acceptance and parental accounts of fussy eating were not related to children's BMI. CONCLUSION: Food texture but not taste or colour alternations affected food acceptance, at least when consuming variations of a well-liked yoghurt. This knowledge is important for further research on picky-eating interventions. Parental reports of fussy eating did not concur with the behavioural observation of food acceptance. Further research is warranted to test whether these findings generalize to other food types.