TY - JOUR
T1 - Biomimetic Mechanically Strong One-Dimensional Hydroxyapatite/Poly(d,l-lactide) Composite Inducing Formation of Anisotropic Collagen Matrix
AU - Zhang, Yonggang
AU - Li, Jiaping
AU - Mouser, Vivian Hilda Maria
AU - Roumans, Nadia
AU - Moroni, Lorenzo
AU - Habibovic, Pamela
N1 - Funding Information:
This research was financially supported by the Gravitation Program “Materials Driven Regeneration”, funded by The Dutch Research Council NWO (Grant #024.003.013). JL, LM and PH acknowledge the financial support by the Dutch Research Council, Applied and Engineering Sciences NWO-AES (Grant #16711). The authors acknowledge Noah Avraham and Prof. Regina Palkovits (RWTH Aachen University, Germany) for assisting with BET measurement.
Publisher Copyright:
©
PY - 2021/11/23
Y1 - 2021/11/23
N2 - Natural bone is a complex composite, consisting predominantly of collagen and hydroxyapatite (HA), which form a highly organized, hierarchical structure from the nano- to the macroscale. Because of its biphasic, anisotropic, ultrafine structural design, bone tissue possesses excellent mechanical properties. Herein, inspired by the composition and microstructure of natural bone, a biphasic composite consisting of highly aligned strontium/copper-doped one-dimensional hydroxyapatite (Sr/Cu-doped 1D HA) and poly(d,l-lactide) (PDLA) was developed. The presence and alignment of Sr/Cu-doped 1D HA crystals resulted in mechanical reinforcement of the polymer matrix, including compressive and tensile strength and modulus, fracture toughness, swelling resistance, and long-term structural stability. The compressive strength, tensile strength, and Young's modulus of the biomimetic composite were comparable to that of cortical bone. Biologically, the biomimetic composite showed a sustained release of the incorporated Sr and Cu ions, facilitated mineral deposition from simulated body fluid, and supported attachment, proliferation, and alkaline phosphatase activity of human mesenchymal stromal cells (hMSCs). Moreover, the highly aligned Sr/Cu-doped 1D HA crystals in the 3D porous scaffolds induced the alignment of hMSCs and secretion of an anisotropic collagen fiber matrix in 3D. The biomimetic Sr/Cu-doped 1D HA/PDLA composite presented here contributes to the current efforts aiming at the design and development of load-bearing bioactive synthetic bone graft substitutes. Moreover, the biomimetic composite may serve as a 3D platform for studying cell-extracellular matrix interactions in bone tissue.
AB - Natural bone is a complex composite, consisting predominantly of collagen and hydroxyapatite (HA), which form a highly organized, hierarchical structure from the nano- to the macroscale. Because of its biphasic, anisotropic, ultrafine structural design, bone tissue possesses excellent mechanical properties. Herein, inspired by the composition and microstructure of natural bone, a biphasic composite consisting of highly aligned strontium/copper-doped one-dimensional hydroxyapatite (Sr/Cu-doped 1D HA) and poly(d,l-lactide) (PDLA) was developed. The presence and alignment of Sr/Cu-doped 1D HA crystals resulted in mechanical reinforcement of the polymer matrix, including compressive and tensile strength and modulus, fracture toughness, swelling resistance, and long-term structural stability. The compressive strength, tensile strength, and Young's modulus of the biomimetic composite were comparable to that of cortical bone. Biologically, the biomimetic composite showed a sustained release of the incorporated Sr and Cu ions, facilitated mineral deposition from simulated body fluid, and supported attachment, proliferation, and alkaline phosphatase activity of human mesenchymal stromal cells (hMSCs). Moreover, the highly aligned Sr/Cu-doped 1D HA crystals in the 3D porous scaffolds induced the alignment of hMSCs and secretion of an anisotropic collagen fiber matrix in 3D. The biomimetic Sr/Cu-doped 1D HA/PDLA composite presented here contributes to the current efforts aiming at the design and development of load-bearing bioactive synthetic bone graft substitutes. Moreover, the biomimetic composite may serve as a 3D platform for studying cell-extracellular matrix interactions in bone tissue.
KW - ANGIOGENESIS
KW - BIOACTIVE GLASS SCAFFOLDS
KW - BONE
KW - CALCIUM-PHOSPHATE
KW - DIFFERENTIATION
KW - HYDROXYAPATITE
KW - IN-VITRO
KW - MESENCHYMAL STEM-CELLS
KW - OF-THE-ART
KW - OSTEOBLAST-LIKE CELLS
KW - PDLA
KW - anisotropy
KW - biomimetic
KW - bone
KW - composite
KW - hydroxyapatite
U2 - 10.1021/acsnano.1c03905
DO - 10.1021/acsnano.1c03905
M3 - Article
C2 - 34662097
SN - 1936-0851
VL - 15
SP - 17480
EP - 17498
JO - ACS Nano
JF - ACS Nano
IS - 11
ER -