TY - JOUR
T1 - Biomimetic Bacterial Identification Platform Based on Thermal Wave Transport Analysis (TWTA) through Surface-Imprinted Polymers
AU - Redeker, Erik Steen
AU - Eersels, Kasper
AU - Akkermans, Onno
AU - Royakkers, Jeroen
AU - Dyson, Simba
AU - Nurekeyeva, Kunya
AU - Ferrando, Beniamino
AU - Cornelis, Peter
AU - Peeters, Marloes
AU - Wagner, Patrick
AU - Dilien, Hanne
AU - van Grinsven, Bart
AU - Cleij, Thomas Jan
PY - 2017/5
Y1 - 2017/5
N2 - This paper introduces a novel bacterial identification assay based on thermal wave analysis through surface imprinted polymers (SIPs). Aluminum chips are coated with SIPs, serving as synthetic cell receptors that have been combined previously with the heat-transfer method (HTM) for the selective detection of bacteria. In this work, the concept of bacterial identification is extended toward the detection of nine different bacterial species. In addition, a novel sensing approach, thermal wave transport analysis (TWTA), is introduced, which analyzes the propagation of a thermal wave through a functional interface. The results presented here demonstrate that bacterial rebinding to the SIP layer resulted in a measurable phase shift in the propagated wave, which is most pronounced at a frequency of 0.03 Hz. In this way, the sensor is able to selectively distinguish between the different bacterial species used in this study. Furthermore, a dose response curve was constructed to determine a limit of detection of 1 x 10(4) CFU mL(-1), indicating that TWTA is advantageous over HTM in terms of sensitivity and response time. Additionally, the limit of selectivity of the sensor was tested in a mixed bacterial solution, containing the target species in the presence of a 99-fold excess of competitor species. Finally, a first application for the sensor in terms of infection diagnosis is presented, revealing that the platform is able to detect bacteria in clinically relevant concentrations as low as 3 X 10(4) CFU mL-1 in spiked urine samples.
AB - This paper introduces a novel bacterial identification assay based on thermal wave analysis through surface imprinted polymers (SIPs). Aluminum chips are coated with SIPs, serving as synthetic cell receptors that have been combined previously with the heat-transfer method (HTM) for the selective detection of bacteria. In this work, the concept of bacterial identification is extended toward the detection of nine different bacterial species. In addition, a novel sensing approach, thermal wave transport analysis (TWTA), is introduced, which analyzes the propagation of a thermal wave through a functional interface. The results presented here demonstrate that bacterial rebinding to the SIP layer resulted in a measurable phase shift in the propagated wave, which is most pronounced at a frequency of 0.03 Hz. In this way, the sensor is able to selectively distinguish between the different bacterial species used in this study. Furthermore, a dose response curve was constructed to determine a limit of detection of 1 x 10(4) CFU mL(-1), indicating that TWTA is advantageous over HTM in terms of sensitivity and response time. Additionally, the limit of selectivity of the sensor was tested in a mixed bacterial solution, containing the target species in the presence of a 99-fold excess of competitor species. Finally, a first application for the sensor in terms of infection diagnosis is presented, revealing that the platform is able to detect bacteria in clinically relevant concentrations as low as 3 X 10(4) CFU mL-1 in spiked urine samples.
KW - surface-imprinted polymers
KW - thermal wave transport analysis (TWTA)
KW - bacterial identification
KW - cross-selectivity matrix
KW - mixed bacterial solution
U2 - 10.1021/acsinfecdis.7b00037
DO - 10.1021/acsinfecdis.7b00037
M3 - Article
C2 - 28388095
SN - 2373-8227
VL - 3
SP - 388
EP - 397
JO - ACS Infectious Diseases
JF - ACS Infectious Diseases
IS - 5
ER -