TY - JOUR
T1 - Biological variation in tPA-induced plasma clot lysis time
AU - Talens, Simone
AU - Malfliet, Joyce J. M. C.
AU - Rudez, Goran
AU - Spronk, Henri M. H.
AU - Janssen, Nicole A. H.
AU - Meijer, Piet
AU - Kluft, Cornelis
AU - de Maat, Moniek P. M.
AU - Rijken, Dingeman C.
PY - 2012/10
Y1 - 2012/10
N2 - Hypofibrinolysis is a risk factor for venous and arterial thrombosis, and can be assessed by using a turbidimetric tPA-induced clot lysis time (CLT) assay. Biological variation in clot lysis time may affect the interpretation and usefulness of CLT as a risk factor for thrombosis. Sufficient information about assay variation and biological variation in CLT is not yet available. Thus, this study aimed to determine the analytical, within-subject and between-subject variation in CLT. We collected blood samples from 40 healthy individuals throughout a period of one year (average 11.8 visits) and determined the CLT of each plasma sample in duplicate. The mean (+/- SD) CLT was 83.8 (+/- 11.1) minutes. The coefficients of variation for total variation, analytical variation, within-subject variation and between-subject variation were 13.4%, 2.6%, 8.2% and 10.2%, respectively. One measurement can estimate the CLT that does not deviate more than 20% from its true value. The contribution of analytical variation to the within-subject variation was 5.0%, the index of individuality was 0.84 and the reference change value was 23.8%. The CLT was longer in the morning compared to the afternoon and was slightly longer in older individuals (> 40 years) compared to younger (
AB - Hypofibrinolysis is a risk factor for venous and arterial thrombosis, and can be assessed by using a turbidimetric tPA-induced clot lysis time (CLT) assay. Biological variation in clot lysis time may affect the interpretation and usefulness of CLT as a risk factor for thrombosis. Sufficient information about assay variation and biological variation in CLT is not yet available. Thus, this study aimed to determine the analytical, within-subject and between-subject variation in CLT. We collected blood samples from 40 healthy individuals throughout a period of one year (average 11.8 visits) and determined the CLT of each plasma sample in duplicate. The mean (+/- SD) CLT was 83.8 (+/- 11.1) minutes. The coefficients of variation for total variation, analytical variation, within-subject variation and between-subject variation were 13.4%, 2.6%, 8.2% and 10.2%, respectively. One measurement can estimate the CLT that does not deviate more than 20% from its true value. The contribution of analytical variation to the within-subject variation was 5.0%, the index of individuality was 0.84 and the reference change value was 23.8%. The CLT was longer in the morning compared to the afternoon and was slightly longer in older individuals (> 40 years) compared to younger (
KW - CLT
KW - biological variation
KW - fibrinolysis
U2 - 10.1160/TH12-02-0124
DO - 10.1160/TH12-02-0124
M3 - Article
C2 - 22836204
SN - 0340-6245
VL - 108
SP - 640
EP - 646
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
IS - 4
ER -