Biocatalytic microgels (μ-Gelzymes): synthesis, concepts, and emerging applications

Maximilian Noeth, Elisabeth Gau, Falco Jung, Mehdi D. Davari, Islam El-Awaad*, Andrij Pich*, Ulrich Schwaneberg*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

11 Citations (Web of Science)

Abstract

Enzymes are nature's catalysts and have great potential for sustainable processes from renewable resources. The adaptation of enzymes to the requirements of industrial processes is a prerequisite to harness their benefits in large-scale transformations for the synthesis of fine chemicals, food products, and pharmaceuticals. Immobilisation of wild-type or engineered enzymes using natural and synthetic carriers has been extensively employed to improve catalytic performance, ensure recovery and reuse, and thereby promote their use in industrial processes. Over the past three decades, significant progress has been achieved in the design and application of immobilised enzymes. Microgels as containers for protein immobilisation are advancing into a promising alternative as reflected by a growing body of literature that documents their use in sustainable catalytic processes. Microgels are crosslinked submicron-sized colloidal polymer networks and in this review, we summarise the progress in the synthesis and applications of enzyme-loaded microgels (mu-Gelzymes). We start by exploring the different approaches used for enzyme immobilisation on or within microgels and give representative examples for the use of mu-Gelzymes in different applications. Subsequently, the potential of mu-Gelzymes in achieving sustainable catalysis is discussed from a green chemistry perspective. Finally, we draw future directions for further improvement of biocatalytic mu-Gelzymes as an emerging interdisciplinary research field of interactive soft matter.

Original languageEnglish
Pages (from-to)8183-8209
Number of pages27
JournalGreen Chemistry
Volume22
Issue number23
DOIs
Publication statusPublished - 7 Dec 2020

Keywords

  • MESOPOROUS SILICA NANOPARTICLES
  • CRITICAL SOLUTION TEMPERATURE
  • ENZYME IMMOBILIZATION
  • GREEN CHEMISTRY
  • PRECIPITATION POLYMERIZATION
  • HORSERADISH-PEROXIDASE
  • ENVIRONMENTALLY BENIGN
  • BETA-GALACTOSIDASE
  • CANDIDA-ANTARCTICA
  • AQUEOUS MICROGELS

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