Beneficial effects of sitostanol on the attenuated immune function in asthma patients: results of an in vitro approach

F. Brüll, R.P. Mensink, M.F. Steinbusch, C. Husche, D. Lutjohann, G.J. Wesseling, J. Plat

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

BACKGROUND: In vitro and animal studies have suggested that plant sterols and stanols increase cytokine production by T-helper-1 cells. This may be beneficial for patient groups characterized by a T-helper-2 dominant immune response, e.g. asthma patients. (1) to evaluate whether sitostanol induces a T-helper-1 shift in peripheral blood mononuclear cells (PBMCs) from asthma patients, and (2) to unravel the role of regulatory T-cells in this respect. METHODOLOGY/PRINCIPAL FINDINGS: PBMCs from 10 asthma patients and 10 healthy subjects were isolated and incubated with 1.2 microM sitostanol, while stimulated with 5 microg/ml PHA. Similar amounts of cholesterol were used to determine whether effects were specific for plant stanols or for sterols in general. Changes in cytokine production were measured using antibody arrays and ELISAs. Changes in regulatory T-cell population size were measured by flow cytometry, using intracellular Foxp3 staining. Sitostanol increased production of IFNgamma by 6.5% and IL-2 by 6.0% compared to cholesterol (p<0.01). No changes in IL-4 and IL-13 were found. Interestingly, this effect was only present in PBMCs from asthma patients. The number of Foxp3+ cells tended to increase and their activity, measured by IL-10 production, increased after sitostanol treatment in PBMCs from asthma patients compared to controls by 32.3% (p = 0.077) and 13.3% (p<0.05), respectively. CONCLUSIONS/SIGNIFICANCE: Altogether, the sitostanol-induced Thelper-1 shift in PBMCs from asthma patients and the stimulating effects of sitostanol on Treg cell numbers and activity indicate a possible novel approach for plant stanol ester enriched functional foods in the amelioration of asthmatic symptoms. Functional effects, however, require further evaluation.
Original languageEnglish
Article number46895
Number of pages10
JournalPLOS ONE
Volume7
Issue number10
DOIs
Publication statusPublished - 16 Oct 2012

Keywords

  • REGULATORY T-CELLS
  • ALLERGIC AIRWAY INFLAMMATION
  • NATURAL-KILLER-CELLS
  • NKT CELLS
  • PLANT STEROLS
  • PERIPHERAL-BLOOD
  • TLR2 AGONIST
  • MURINE MODEL
  • SIMVASTATIN
  • STANOLS

Cite this

@article{a07094cfa31f46ca83afa8f64fdc925a,
title = "Beneficial effects of sitostanol on the attenuated immune function in asthma patients: results of an in vitro approach",
abstract = "BACKGROUND: In vitro and animal studies have suggested that plant sterols and stanols increase cytokine production by T-helper-1 cells. This may be beneficial for patient groups characterized by a T-helper-2 dominant immune response, e.g. asthma patients. (1) to evaluate whether sitostanol induces a T-helper-1 shift in peripheral blood mononuclear cells (PBMCs) from asthma patients, and (2) to unravel the role of regulatory T-cells in this respect. METHODOLOGY/PRINCIPAL FINDINGS: PBMCs from 10 asthma patients and 10 healthy subjects were isolated and incubated with 1.2 microM sitostanol, while stimulated with 5 microg/ml PHA. Similar amounts of cholesterol were used to determine whether effects were specific for plant stanols or for sterols in general. Changes in cytokine production were measured using antibody arrays and ELISAs. Changes in regulatory T-cell population size were measured by flow cytometry, using intracellular Foxp3 staining. Sitostanol increased production of IFNgamma by 6.5{\%} and IL-2 by 6.0{\%} compared to cholesterol (p<0.01). No changes in IL-4 and IL-13 were found. Interestingly, this effect was only present in PBMCs from asthma patients. The number of Foxp3+ cells tended to increase and their activity, measured by IL-10 production, increased after sitostanol treatment in PBMCs from asthma patients compared to controls by 32.3{\%} (p = 0.077) and 13.3{\%} (p<0.05), respectively. CONCLUSIONS/SIGNIFICANCE: Altogether, the sitostanol-induced Thelper-1 shift in PBMCs from asthma patients and the stimulating effects of sitostanol on Treg cell numbers and activity indicate a possible novel approach for plant stanol ester enriched functional foods in the amelioration of asthmatic symptoms. Functional effects, however, require further evaluation.",
keywords = "REGULATORY T-CELLS, ALLERGIC AIRWAY INFLAMMATION, NATURAL-KILLER-CELLS, NKT CELLS, PLANT STEROLS, PERIPHERAL-BLOOD, TLR2 AGONIST, MURINE MODEL, SIMVASTATIN, STANOLS",
author = "F. Br{\"u}ll and R.P. Mensink and M.F. Steinbusch and C. Husche and D. Lutjohann and G.J. Wesseling and J. Plat",
year = "2012",
month = "10",
day = "16",
doi = "10.1371/journal.pone.0046895",
language = "English",
volume = "7",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

Beneficial effects of sitostanol on the attenuated immune function in asthma patients: results of an in vitro approach. / Brüll, F.; Mensink, R.P.; Steinbusch, M.F.; Husche, C.; Lutjohann, D.; Wesseling, G.J.; Plat, J.

In: PLOS ONE, Vol. 7, No. 10, 46895, 16.10.2012.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Beneficial effects of sitostanol on the attenuated immune function in asthma patients: results of an in vitro approach

AU - Brüll, F.

AU - Mensink, R.P.

AU - Steinbusch, M.F.

AU - Husche, C.

AU - Lutjohann, D.

AU - Wesseling, G.J.

AU - Plat, J.

PY - 2012/10/16

Y1 - 2012/10/16

N2 - BACKGROUND: In vitro and animal studies have suggested that plant sterols and stanols increase cytokine production by T-helper-1 cells. This may be beneficial for patient groups characterized by a T-helper-2 dominant immune response, e.g. asthma patients. (1) to evaluate whether sitostanol induces a T-helper-1 shift in peripheral blood mononuclear cells (PBMCs) from asthma patients, and (2) to unravel the role of regulatory T-cells in this respect. METHODOLOGY/PRINCIPAL FINDINGS: PBMCs from 10 asthma patients and 10 healthy subjects were isolated and incubated with 1.2 microM sitostanol, while stimulated with 5 microg/ml PHA. Similar amounts of cholesterol were used to determine whether effects were specific for plant stanols or for sterols in general. Changes in cytokine production were measured using antibody arrays and ELISAs. Changes in regulatory T-cell population size were measured by flow cytometry, using intracellular Foxp3 staining. Sitostanol increased production of IFNgamma by 6.5% and IL-2 by 6.0% compared to cholesterol (p<0.01). No changes in IL-4 and IL-13 were found. Interestingly, this effect was only present in PBMCs from asthma patients. The number of Foxp3+ cells tended to increase and their activity, measured by IL-10 production, increased after sitostanol treatment in PBMCs from asthma patients compared to controls by 32.3% (p = 0.077) and 13.3% (p<0.05), respectively. CONCLUSIONS/SIGNIFICANCE: Altogether, the sitostanol-induced Thelper-1 shift in PBMCs from asthma patients and the stimulating effects of sitostanol on Treg cell numbers and activity indicate a possible novel approach for plant stanol ester enriched functional foods in the amelioration of asthmatic symptoms. Functional effects, however, require further evaluation.

AB - BACKGROUND: In vitro and animal studies have suggested that plant sterols and stanols increase cytokine production by T-helper-1 cells. This may be beneficial for patient groups characterized by a T-helper-2 dominant immune response, e.g. asthma patients. (1) to evaluate whether sitostanol induces a T-helper-1 shift in peripheral blood mononuclear cells (PBMCs) from asthma patients, and (2) to unravel the role of regulatory T-cells in this respect. METHODOLOGY/PRINCIPAL FINDINGS: PBMCs from 10 asthma patients and 10 healthy subjects were isolated and incubated with 1.2 microM sitostanol, while stimulated with 5 microg/ml PHA. Similar amounts of cholesterol were used to determine whether effects were specific for plant stanols or for sterols in general. Changes in cytokine production were measured using antibody arrays and ELISAs. Changes in regulatory T-cell population size were measured by flow cytometry, using intracellular Foxp3 staining. Sitostanol increased production of IFNgamma by 6.5% and IL-2 by 6.0% compared to cholesterol (p<0.01). No changes in IL-4 and IL-13 were found. Interestingly, this effect was only present in PBMCs from asthma patients. The number of Foxp3+ cells tended to increase and their activity, measured by IL-10 production, increased after sitostanol treatment in PBMCs from asthma patients compared to controls by 32.3% (p = 0.077) and 13.3% (p<0.05), respectively. CONCLUSIONS/SIGNIFICANCE: Altogether, the sitostanol-induced Thelper-1 shift in PBMCs from asthma patients and the stimulating effects of sitostanol on Treg cell numbers and activity indicate a possible novel approach for plant stanol ester enriched functional foods in the amelioration of asthmatic symptoms. Functional effects, however, require further evaluation.

KW - REGULATORY T-CELLS

KW - ALLERGIC AIRWAY INFLAMMATION

KW - NATURAL-KILLER-CELLS

KW - NKT CELLS

KW - PLANT STEROLS

KW - PERIPHERAL-BLOOD

KW - TLR2 AGONIST

KW - MURINE MODEL

KW - SIMVASTATIN

KW - STANOLS

U2 - 10.1371/journal.pone.0046895

DO - 10.1371/journal.pone.0046895

M3 - Article

VL - 7

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 10

M1 - 46895

ER -