Belgian recommendations on ANA, anti-dsDNA and anti-ENA antibody testing

M. Van Blerk; X. Bossuyt; R. Humbel; A. Mewis; G. Servais; J. P. Tomasi; C. Van Campenhout; L. Van Hoovels; M. Vercammen; J. Damoiseaux; W. Coucke; P. Van de Walle

doi:10.1179/2295333714Y.0000000010

Belgian recommendations on ANA, anti-dsDNA and anti-ENA antibody testing

M. Van Blerk^*, X. Bossuyt, R. Humbel, A. Mewis, G. Servais, J. P. Tomasi, C. Van Campenhout, L. Van Hoovels, M. Vercammen, J. Damoiseaux, W. Coucke, P. Van de Walle

^*Corresponding author for this work

DA CDL Algemeen

Research output: Contribution to journal › Article › Academic › peer-review

11 Citations (Web of Science)

Abstract

Autoantibodies to nuclear antigens, i.e. antinuclear antibodies (ANA), antibodies to double-stranded DNA (dsDNA) and extractable nuclear antigens (ENA), are useful as diagnostic markers for a variety of autoimmune diseases. In March 2010, the Belgian national External Quality Assessment Scheme sent a questionnaire on ANA, anti-dsDNA and anti-ENA antibody testing designed by the Dutch EASI (European Autoimmunity Standardization Initiative) team, to all clinical laboratories performing ANA testing. Virtually all laboratories completed the questionnaire (97.7%, 127/130). This paper discusses the results of this questionnaire and provides valuable information on the state-of-the-art of ANA, anti-dsDNA and anti-ENA antibody testing as practiced in the Belgian laboratories. In addition, this work presents practical recommendations developed by the members of the advisory board of the scheme as a result of the outcome of this study.

Original language	English
Pages (from-to)	83-86
Journal	Acta Clinica Belgica
Volume	69
Issue number	2
DOIs	https://doi.org/10.1179/2295333714Y.0000000010
Publication status	Published - Apr 2014

Keywords

Antinuclear antibodies
Anti-dsDNA antibodies
Anti-ENA antibodies
Recommendations
External quality assessment

Access to Document

10.1179/2295333714Y.0000000010

Cite this

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title = "Belgian recommendations on ANA, anti-dsDNA and anti-ENA antibody testing",

abstract = "Autoantibodies to nuclear antigens, i.e. antinuclear antibodies (ANA), antibodies to double-stranded DNA (dsDNA) and extractable nuclear antigens (ENA), are useful as diagnostic markers for a variety of autoimmune diseases. In March 2010, the Belgian national External Quality Assessment Scheme sent a questionnaire on ANA, anti-dsDNA and anti-ENA antibody testing designed by the Dutch EASI (European Autoimmunity Standardization Initiative) team, to all clinical laboratories performing ANA testing. Virtually all laboratories completed the questionnaire (97.7%, 127/130). This paper discusses the results of this questionnaire and provides valuable information on the state-of-the-art of ANA, anti-dsDNA and anti-ENA antibody testing as practiced in the Belgian laboratories. In addition, this work presents practical recommendations developed by the members of the advisory board of the scheme as a result of the outcome of this study.",

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author = "{Van Blerk}, M. and X. Bossuyt and R. Humbel and A. Mewis and G. Servais and Tomasi, {J. P.} and {Van Campenhout}, C. and {Van Hoovels}, L. and M. Vercammen and J. Damoiseaux and W. Coucke and {Van de Walle}, P.",

year = "2014",

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doi = "10.1179/2295333714Y.0000000010",

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T1 - Belgian recommendations on ANA, anti-dsDNA and anti-ENA antibody testing

AU - Van Blerk, M.

AU - Bossuyt, X.

AU - Humbel, R.

AU - Mewis, A.

AU - Servais, G.

AU - Tomasi, J. P.

AU - Van Campenhout, C.

AU - Van Hoovels, L.

AU - Vercammen, M.

AU - Damoiseaux, J.

AU - Coucke, W.

AU - Van de Walle, P.

PY - 2014/4

Y1 - 2014/4

N2 - Autoantibodies to nuclear antigens, i.e. antinuclear antibodies (ANA), antibodies to double-stranded DNA (dsDNA) and extractable nuclear antigens (ENA), are useful as diagnostic markers for a variety of autoimmune diseases. In March 2010, the Belgian national External Quality Assessment Scheme sent a questionnaire on ANA, anti-dsDNA and anti-ENA antibody testing designed by the Dutch EASI (European Autoimmunity Standardization Initiative) team, to all clinical laboratories performing ANA testing. Virtually all laboratories completed the questionnaire (97.7%, 127/130). This paper discusses the results of this questionnaire and provides valuable information on the state-of-the-art of ANA, anti-dsDNA and anti-ENA antibody testing as practiced in the Belgian laboratories. In addition, this work presents practical recommendations developed by the members of the advisory board of the scheme as a result of the outcome of this study.

AB - Autoantibodies to nuclear antigens, i.e. antinuclear antibodies (ANA), antibodies to double-stranded DNA (dsDNA) and extractable nuclear antigens (ENA), are useful as diagnostic markers for a variety of autoimmune diseases. In March 2010, the Belgian national External Quality Assessment Scheme sent a questionnaire on ANA, anti-dsDNA and anti-ENA antibody testing designed by the Dutch EASI (European Autoimmunity Standardization Initiative) team, to all clinical laboratories performing ANA testing. Virtually all laboratories completed the questionnaire (97.7%, 127/130). This paper discusses the results of this questionnaire and provides valuable information on the state-of-the-art of ANA, anti-dsDNA and anti-ENA antibody testing as practiced in the Belgian laboratories. In addition, this work presents practical recommendations developed by the members of the advisory board of the scheme as a result of the outcome of this study.

KW - Antinuclear antibodies

KW - Anti-dsDNA antibodies

KW - Anti-ENA antibodies

KW - Recommendations

KW - External quality assessment

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DO - 10.1179/2295333714Y.0000000010

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SN - 1784-3286

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