In the present study we evaluated a new method to assess the behavioural and biochemical effects of a brief period of acute hypoxia in the brain. In this method, cyanide is injected into the lateral ventricles. Spatial navigation performance in a Morris task was found to be impaired 1 and 5 min after an i.c.v. injection of 5.0 μg cyanide but not after 2.5 μg cyanide. Increased rate of phosphatidic acid formation, reflecting increased phospholipase C activity, were observed after injection of 5.0 μg cyanide, indicating that energy-dependent phosphoinositide metabolism was affected. Chronic treatment with acetyl-carnitine attenuated the cyanide-induced behavioural deficit, but had no effect on energy-dependent phophoinositide metabolism. The results suggest that, in this model, acetyl-l-carnitine may act via free fatty acid metabolism, by increasing the reservoir of activated acyl groups which are involved in the reacylation of membrane phospholipids.
Blokland, A., Bothmer, J., Honig, W., & Jolles, J. (1993). Behavioural and biochemical effects of acute central metabolic inhibition: Effects of acetyl-l-carnitine. European Journal of Pharmacology, 235(2-3), 275-281. https://doi.org/10.1016/0014-2999(93)90146-9