Beetroot Juice Increases Human Muscle Force without Changing Ca2+-Handling Proteins

Jamie Whitfield; Daniel Gamu; George J. F. Heigenhauser; Luc J. C. Van Loon; Lawrence L. Spriet; A. Russell Tupling; Graham P. Holloway

doi:10.1249/MSS.0000000000001321

Beetroot Juice Increases Human Muscle Force without Changing Ca2+-Handling Proteins

Jamie Whitfield^*, Daniel Gamu, George J. F. Heigenhauser, Luc J. C. Van Loon, Lawrence L. Spriet, A. Russell Tupling, Graham P. Holloway

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Purpose: This study aimed to examine the effects of 7 d of beetroot juice (BRJ) supplementation on SkM contractile characteristics and function. Methods: Recreationally active males (n = 8) underwent transcutaneous electrical muscle stimulation of the vastus lateralis for the evaluation of contractile characteristics before and after 7 d of BRJ supplementation (280 mLIdj1, similar to 26 mmol NO3-). An additional group of individuals (n = 8) followed the same supplementation protocol but underwent SkM biopsies pre and post-supplementation for the determination of proteins associated with calcium handling via Western blotting, and the ratio of reduced/oxidized glutathione (GSH: GSSG), an indicator of cellular redox state, via high-performance liquid chromatography (HPLC). Results: After supplementation, there was no change in maximal voluntary force production (602 +/- 50 vs 596 +/- 56 N) or electrically induced tetanic contractions. By contrast, force production was increased at 10 Hz electrical stimulation (41.1% +/- 2.3% vs 37.6% +/- 2.4% of peak force, P

Original language	English
Pages (from-to)	2016-2024
Number of pages	9
Journal	Medicine and Science in Sports and Exercise
Volume	49
Issue number	10
DOIs	https://doi.org/10.1249/MSS.0000000000001321
Publication status	Published - Oct 2017

Keywords

NITRATE
NITRITE
NITRIC OXIDE
EXCITATION-CONTRACTION COUPLING
MAXIMUM VOLUNTARY CONTRACTION
FORCE-FREQUENCY RELATIONSHIP
DIETARY NITRATE SUPPLEMENTATION
HUMAN SKELETAL-MUSCLE
TIME-TRIAL PERFORMANCE
REACTIVE OXYGEN
MITOCHONDRIAL EFFICIENCY
SARCOPLASMIC-RETICULUM
CONTRACTILE PROPERTIES
VASTUS LATERALIS
BLOOD-PRESSURE
NITRIC-OXIDE

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@article{2c990669d4be483baee6678294ebd988,

title = "Beetroot Juice Increases Human Muscle Force without Changing Ca2+-Handling Proteins",

abstract = "Purpose: This study aimed to examine the effects of 7 d of beetroot juice (BRJ) supplementation on SkM contractile characteristics and function. Methods: Recreationally active males (n = 8) underwent transcutaneous electrical muscle stimulation of the vastus lateralis for the evaluation of contractile characteristics before and after 7 d of BRJ supplementation (280 mLIdj1, similar to 26 mmol NO3-). An additional group of individuals (n = 8) followed the same supplementation protocol but underwent SkM biopsies pre and post-supplementation for the determination of proteins associated with calcium handling via Western blotting, and the ratio of reduced/oxidized glutathione (GSH: GSSG), an indicator of cellular redox state, via high-performance liquid chromatography (HPLC). Results: After supplementation, there was no change in maximal voluntary force production (602 +/- 50 vs 596 +/- 56 N) or electrically induced tetanic contractions. By contrast, force production was increased at 10 Hz electrical stimulation (41.1% +/- 2.3% vs 37.6% +/- 2.4% of peak force, P",

keywords = "NITRATE, NITRITE, NITRIC OXIDE, EXCITATION-CONTRACTION COUPLING, MAXIMUM VOLUNTARY CONTRACTION, FORCE-FREQUENCY RELATIONSHIP, DIETARY NITRATE SUPPLEMENTATION, HUMAN SKELETAL-MUSCLE, TIME-TRIAL PERFORMANCE, REACTIVE OXYGEN, MITOCHONDRIAL EFFICIENCY, SARCOPLASMIC-RETICULUM, CONTRACTILE PROPERTIES, VASTUS LATERALIS, BLOOD-PRESSURE, NITRIC-OXIDE",

author = "Jamie Whitfield and Daniel Gamu and Heigenhauser, {George J. F.} and {Van Loon}, {Luc J. C.} and Spriet, {Lawrence L.} and Tupling, {A. Russell} and Holloway, {Graham P.}",

year = "2017",

month = oct,

doi = "10.1249/MSS.0000000000001321",

language = "English",

volume = "49",

pages = "2016--2024",

journal = "Medicine and Science in Sports and Exercise",

issn = "0195-9131",

publisher = "LIPPINCOTT WILLIAMS & WILKINS",

number = "10",

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T1 - Beetroot Juice Increases Human Muscle Force without Changing Ca2+-Handling Proteins

AU - Whitfield, Jamie

AU - Gamu, Daniel

AU - Heigenhauser, George J. F.

AU - Van Loon, Luc J. C.

AU - Spriet, Lawrence L.

AU - Tupling, A. Russell

AU - Holloway, Graham P.

PY - 2017/10

Y1 - 2017/10

N2 - Purpose: This study aimed to examine the effects of 7 d of beetroot juice (BRJ) supplementation on SkM contractile characteristics and function. Methods: Recreationally active males (n = 8) underwent transcutaneous electrical muscle stimulation of the vastus lateralis for the evaluation of contractile characteristics before and after 7 d of BRJ supplementation (280 mLIdj1, similar to 26 mmol NO3-). An additional group of individuals (n = 8) followed the same supplementation protocol but underwent SkM biopsies pre and post-supplementation for the determination of proteins associated with calcium handling via Western blotting, and the ratio of reduced/oxidized glutathione (GSH: GSSG), an indicator of cellular redox state, via high-performance liquid chromatography (HPLC). Results: After supplementation, there was no change in maximal voluntary force production (602 +/- 50 vs 596 +/- 56 N) or electrically induced tetanic contractions. By contrast, force production was increased at 10 Hz electrical stimulation (41.1% +/- 2.3% vs 37.6% +/- 2.4% of peak force, P

AB - Purpose: This study aimed to examine the effects of 7 d of beetroot juice (BRJ) supplementation on SkM contractile characteristics and function. Methods: Recreationally active males (n = 8) underwent transcutaneous electrical muscle stimulation of the vastus lateralis for the evaluation of contractile characteristics before and after 7 d of BRJ supplementation (280 mLIdj1, similar to 26 mmol NO3-). An additional group of individuals (n = 8) followed the same supplementation protocol but underwent SkM biopsies pre and post-supplementation for the determination of proteins associated with calcium handling via Western blotting, and the ratio of reduced/oxidized glutathione (GSH: GSSG), an indicator of cellular redox state, via high-performance liquid chromatography (HPLC). Results: After supplementation, there was no change in maximal voluntary force production (602 +/- 50 vs 596 +/- 56 N) or electrically induced tetanic contractions. By contrast, force production was increased at 10 Hz electrical stimulation (41.1% +/- 2.3% vs 37.6% +/- 2.4% of peak force, P

KW - NITRATE

KW - NITRITE

KW - NITRIC OXIDE

KW - EXCITATION-CONTRACTION COUPLING

KW - MAXIMUM VOLUNTARY CONTRACTION

KW - FORCE-FREQUENCY RELATIONSHIP

KW - DIETARY NITRATE SUPPLEMENTATION

KW - HUMAN SKELETAL-MUSCLE

KW - TIME-TRIAL PERFORMANCE

KW - REACTIVE OXYGEN

KW - MITOCHONDRIAL EFFICIENCY

KW - SARCOPLASMIC-RETICULUM

KW - CONTRACTILE PROPERTIES

KW - VASTUS LATERALIS

KW - BLOOD-PRESSURE

KW - NITRIC-OXIDE

U2 - 10.1249/MSS.0000000000001321

DO - 10.1249/MSS.0000000000001321

M3 - Article

SN - 0195-9131

VL - 49

SP - 2016

EP - 2024

JO - Medicine and Science in Sports and Exercise

JF - Medicine and Science in Sports and Exercise

IS - 10

ER -

Beetroot Juice Increases Human Muscle Force without Changing Ca2+-Handling Proteins

Abstract

Keywords

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