Bactericidal/permeability-increasing protein release in whole blood ex vivo: strong induction by lipopolysaccharide and tumor necrosis factor-alpha.

M.A. Dentener, G.J.M. Francot, P.S. Hiemstra, A.T.J. Tool, A.J. Verhoeven, P. Vandenabeele, W.A. Buurman

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Department of Pulmonology, University of Limburg, Maastricht, Netherlands.

In this study, the release of bactericidal/permeability-increasing protein (BPI), which is stored in polymorphonuclear leukocytes (PMNL), was analyzed in a whole blood ex vivo system. Of the microbial products tested, lipopolysaccharide (LPS) most potently induced BPI release; FMLP, serum-treated zymosan (STZ), and lipoteichoic acid (LTA) also induced BPI release. In addition, the inflammatory mediator tumor necrosis factor (TNF)-alpha potently activated PMNL in whole blood, via TNF receptor p55, to release BPI, whereas interleukin (IL)-1, IL-8, platelet activating factor, and C5a were poor inducers of BPI release. STZ and phorbol myristate acetate, but not LPS, FMLP, or LTA, stimulated isolated PMNL to release BPI. BPI was released in comparable magnitude with the azurophilic granule protein elastase. Furthermore, both proteins were released with similar kinetics, which started within 30 min after onset of stimulation and lasted 1-4 h.
Original languageEnglish
Pages (from-to)108-117
Number of pages10
JournalJournal of Infectious Diseases
Volume175
Issue number1
DOIs
Publication statusPublished - 1 Jan 1997

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