Sample preparation for lipid analysis of intra-articular adipose tissue by using matrix-assisted laser desorption/ionization imaging

Mirella J J Haartmans; Britt S R Claes; Kaj S Emanuel; Gabrielle J M Tuijthof; Ron M A Heeren; Pieter J Emans; Berta Cillero-Pastor

doi:10.1016/j.ab.2022.115018

Sample preparation for lipid analysis of intra-articular adipose tissue by using matrix-assisted laser desorption/ionization imaging

Mirella J J Haartmans, Britt S R Claes, Kaj S Emanuel, Gabrielle J M Tuijthof, Ron M A Heeren, Pieter J Emans, Berta Cillero-Pastor^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Mass spectrometry imaging (MSI) is a powerful technique enabling the visualization of the spatial distribution of different molecules in tissue biopsies with different pathologies. Sample handling and preparing adipose tissue for MSI is challenging and prone to molecular delocalization due to tissue melting. In this work, we developed a method for matrix-assisted laser desorption/ionization (MALDI)-MSI to study lipids in human infrapatellar fat pad (IPFP), a biomarker source in musculoskeletal pathologies, while preserving molecular spatial distribution. Cryosectioning at 15 μm with a temperature below -30 °C, thaw-mounting, and sublimation, was demonstrated to preserve IPFP's heterogeneous appearance and spatial distribution of lipids.

Original language	English
Article number	115018
Number of pages	5
Journal	Analytical Biochemistry
Volume	662
Issue number	1
Early online date	12 Dec 2022
DOIs	https://doi.org/10.1016/j.ab.2022.115018
Publication status	Published - 1 Feb 2023

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10.1016/j.ab.2022.115018Licence: CC BY

Cite this

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title = "Sample preparation for lipid analysis of intra-articular adipose tissue by using matrix-assisted laser desorption/ionization imaging",

abstract = "Mass spectrometry imaging (MSI) is a powerful technique enabling the visualization of the spatial distribution of different molecules in tissue biopsies with different pathologies. Sample handling and preparing adipose tissue for MSI is challenging and prone to molecular delocalization due to tissue melting. In this work, we developed a method for matrix-assisted laser desorption/ionization (MALDI)-MSI to study lipids in human infrapatellar fat pad (IPFP), a biomarker source in musculoskeletal pathologies, while preserving molecular spatial distribution. Cryosectioning at 15 μm with a temperature below -30 °C, thaw-mounting, and sublimation, was demonstrated to preserve IPFP's heterogeneous appearance and spatial distribution of lipids.",

author = "Haartmans, {Mirella J J} and Claes, {Britt S R} and Emanuel, {Kaj S} and Tuijthof, {Gabrielle J M} and Heeren, {Ron M A} and Emans, {Pieter J} and Berta Cillero-Pastor",

note = "Copyright {\textcopyright} 2022. Published by Elsevier Inc.",

year = "2023",

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doi = "10.1016/j.ab.2022.115018",

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T1 - Sample preparation for lipid analysis of intra-articular adipose tissue by using matrix-assisted laser desorption/ionization imaging

AU - Haartmans, Mirella J J

AU - Claes, Britt S R

AU - Emanuel, Kaj S

AU - Tuijthof, Gabrielle J M

AU - Heeren, Ron M A

AU - Emans, Pieter J

AU - Cillero-Pastor, Berta

PY - 2023/2/1

Y1 - 2023/2/1

N2 - Mass spectrometry imaging (MSI) is a powerful technique enabling the visualization of the spatial distribution of different molecules in tissue biopsies with different pathologies. Sample handling and preparing adipose tissue for MSI is challenging and prone to molecular delocalization due to tissue melting. In this work, we developed a method for matrix-assisted laser desorption/ionization (MALDI)-MSI to study lipids in human infrapatellar fat pad (IPFP), a biomarker source in musculoskeletal pathologies, while preserving molecular spatial distribution. Cryosectioning at 15 μm with a temperature below -30 °C, thaw-mounting, and sublimation, was demonstrated to preserve IPFP's heterogeneous appearance and spatial distribution of lipids.

AB - Mass spectrometry imaging (MSI) is a powerful technique enabling the visualization of the spatial distribution of different molecules in tissue biopsies with different pathologies. Sample handling and preparing adipose tissue for MSI is challenging and prone to molecular delocalization due to tissue melting. In this work, we developed a method for matrix-assisted laser desorption/ionization (MALDI)-MSI to study lipids in human infrapatellar fat pad (IPFP), a biomarker source in musculoskeletal pathologies, while preserving molecular spatial distribution. Cryosectioning at 15 μm with a temperature below -30 °C, thaw-mounting, and sublimation, was demonstrated to preserve IPFP's heterogeneous appearance and spatial distribution of lipids.

U2 - 10.1016/j.ab.2022.115018

DO - 10.1016/j.ab.2022.115018

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

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