@article{b8cbe2ac8de74377bf03696a2772d427,
title = "Sphingolipids control dermal fibroblast heterogeneity",
abstract = "Human cells produce thousands of lipids that change during cell differentiation and can vary across individual cells of the same type. However, we are only starting to characterize the function of these cell-to-cell differences in lipid composition. Here, we measured the lipidomes and transcriptomes of individual human dermal fibroblasts by coupling high-resolution mass spectrometry imaging with single-cell transcriptomics. We found that the cell-to-cell variations of specific lipid metabolic pathways contribute to the establishment of cell states involved in the organization of skin architecture. Sphingolipid composition is shown to define fibroblast subpopulations, with sphingolipid metabolic rewiring driving cell-state transitions. Therefore, cell-to-cell lipid heterogeneity affects the determination of cell states, adding a new regulatory component to the self-organization of multicellular systems.",
keywords = "Fibroblasts/metabolism, Humans, Metabolic Networks and Pathways, Skin, Sphingolipids/chemistry, Transcriptome, REGENERATION, TISSUE, IDENTIFICATION, MASS-SPECTROMETRY, ORGANIZATION, VARIABILITY, INHIBITION, SINGLE-CELL ANALYSIS, EXPRESSION, LIPID EXTRACTION",
author = "Laura Capolupo and Irina Khven and Lederer, {Alex R} and Luigi Mazzeo and Galina Glousker and Sylvia Ho and Francesco Russo and Montoya, {Jonathan Paz} and Bhandari, {Dhaka R} and Bowman, {Andrew P} and Ellis, {Shane R} and Romain Guiet and Olivier Burri and Johanna Detzner and Johannes Muthing and Krisztian Homicsko and Fran{\c c}ois Kuonen and Michel Gilliet and Bernhard Spengler and Heeren, {Ron M A} and Dotto, {G Paolo} and {La Manno}, Gioele and Giovanni D'Angelo",
note = "Funding Information: G.L.M. and I.K. were supported by the Swiss National Science Foundation (grants CRSK-3_190495 and PZ00P3_193445). G.L.M. was supported by the School of Life Sciences, EPFL. G.D. acknowledges financial support from the Swiss Cancer League (grant KFS-4999-02-2020), the EPFL institutional fund, the Kristian Gerhard Jebsen Foundation, and the Swiss National Science Foundation (SNSF grant 310030_184926). L.M. and G.P.D. were supported by the Swiss National Science Foundation (grant 310030B_176404), the National Institutes of Health (NIH grant R01AR039190; the content does not necessarily represent the official views of the NIH), and the European Union?s Horizon 2020 research and innovation program (Marie Sk?odowska-Curie grant 859860). Publisher Copyright: Copyright {\textcopyright} 2022 The Authors, some rights reserved.",
year = "2022",
month = apr,
day = "15",
doi = "10.1126/science.abh1623",
language = "English",
volume = "376",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6590",
}