Automated, parallel mass spectrometry imaging and structural identification of lipids

Shane R. Ellis; Martin R. L. Paine; Gert B. Eijkel; Josch K. Pauling; Peter Husen; Mark W. Jervelund; Martin Hermansson; Christer S. Ejsing; Ron M. A. Heeren

doi:10.1038/s41592-018-0010-6

Automated, parallel mass spectrometry imaging and structural identification of lipids

Shane R. Ellis
, Martin R. L. Paine
, Gert B. Eijkel
, Josch K. Pauling
, Peter Husen
, Mark W. Jervelund
, Martin Hermansson
, Christer S. Ejsing^*
, Ron M. A. Heeren^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.

Original language	English
Pages (from-to)	515–518
Number of pages	4
Journal	Nature Methods
Volume	15
Issue number	7
DOIs	https://doi.org/10.1038/s41592-018-0010-6
Publication status	Published - Jul 2018

Keywords

DATA-ACQUISITION
MALDI
RESOLUTION
INFORMATION
STRATEGIES
LIPIDOMICS
TISSUE
MS

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10.1038/s41592-018-0010-6

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title = "Automated, parallel mass spectrometry imaging and structural identification of lipids",

abstract = "We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.",

keywords = "DATA-ACQUISITION, MALDI, RESOLUTION, INFORMATION, STRATEGIES, LIPIDOMICS, TISSUE, MS",

author = "Ellis, \{Shane R.\} and Paine, \{Martin R. L.\} and Eijkel, \{Gert B.\} and Pauling, \{Josch K.\} and Peter Husen and Jervelund, \{Mark W.\} and Martin Hermansson and Ejsing, \{Christer S.\} and Heeren, \{Ron M. A.\}",

year = "2018",

month = jul,

doi = "10.1038/s41592-018-0010-6",

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T1 - Automated, parallel mass spectrometry imaging and structural identification of lipids

AU - Ellis, Shane R.

AU - Paine, Martin R. L.

AU - Eijkel, Gert B.

AU - Pauling, Josch K.

AU - Husen, Peter

AU - Jervelund, Mark W.

AU - Hermansson, Martin

AU - Ejsing, Christer S.

AU - Heeren, Ron M. A.

PY - 2018/7

Y1 - 2018/7

N2 - We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.

AB - We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.

KW - DATA-ACQUISITION

KW - MALDI

KW - RESOLUTION

KW - INFORMATION

KW - STRATEGIES

KW - LIPIDOMICS

KW - TISSUE

KW - MS

U2 - 10.1038/s41592-018-0010-6

DO - 10.1038/s41592-018-0010-6

M3 - Article

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SN - 1548-7091

VL - 15

SP - 515

EP - 518

JO - Nature Methods

JF - Nature Methods

IS - 7

ER -

Automated, parallel mass spectrometry imaging and structural identification of lipids

Abstract

Keywords

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