Automated, parallel mass spectrometry imaging and structural identification of lipids

Shane R. Ellis, Martin R. L. Paine, Gert B. Eijkel, Josch K. Pauling, Peter Husen, Mark W. Jervelund, Martin Hermansson, Christer S. Ejsing*, Ron M. A. Heeren*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.

Original languageEnglish
Pages (from-to)515–518
Number of pages4
JournalNature Methods
Volume15
Issue number7
DOIs
Publication statusPublished - Jul 2018

Keywords

  • DATA-ACQUISITION
  • MALDI
  • RESOLUTION
  • INFORMATION
  • STRATEGIES
  • LIPIDOMICS
  • TISSUE
  • MS

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