Abstract
We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.
Original language | English |
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Pages (from-to) | 515–518 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 15 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jul 2018 |
Keywords
- DATA-ACQUISITION
- MALDI
- RESOLUTION
- INFORMATION
- STRATEGIES
- LIPIDOMICS
- TISSUE
- MS