Association of Kidney Function with Changes in the Endothelial Surface Layer

Martijn J. C. Dane*, Meriem Khairoun, Dae Hyun Lee, Bernard M. van den Berg, Bart J. M. Eskens, Margien G. S. Boels, Jurgen W. G. E. van Teeffelen, Angelique L. W. M. M. Rops, Johan van der Vlag, Anton Jan van Zonneveld, Marlies E. J. Reinders, Hans Vink, Ton J. Rabelink

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Background and objectivesESRD is accompanied by endothelial dysfunction. Because the endothelial glycocalyx (endothelial surface layer) governs interactions between flowing blood and the vessel wall, perturbation could influence disease progression. This study used a novel noninvasive sidestream-darkfield imaging method, which measures the accessibility of red blood cells to the endothelial surface layer in the microcirculation (perfused boundary region), to investigate whether renal function is associated with endothelial surface layer dimensions.Design, setting, participants, & measurementsPerfused boundary region was measured in control participants (n=10), patients with ESRD (n=23), participants with normal kidney function after successful living donor kidney transplantation (n=12), and patients who developed interstitial fibrosis/tubular atrophy after kidney transplantation (n=10). In addition, the endothelial activation marker angiopoietin-2 and shed endothelial surface layer components syndecan-1 and soluble thrombomodulin were measured using ELISA.ResultsCompared with healthy controls (1.820.16 mu m), ESRD patients had a larger perfused boundary region (+0.23; 95% confidence interval, 0.46 to
Original languageEnglish
Pages (from-to)698-704
JournalClinical journal of the American Society of Nephrology
Volume9
Issue number4
DOIs
Publication statusPublished - 7 Apr 2014

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