Application of high resolution OCT to evaluate irregularity of Bowman's layer in asymmetric keratoconus

Irregularity of the Bowman's layer (BL) in keratoconus (KC, sample size (n) = 28) and forme fruste keratoconus (FFKC, n = 18) eyes was evaluated. Subjects underwent high resolution OCT imaging (Bioptigen Inc., USA) and corneal tomography (Pentacam v1.20r41, OCULUS Optikgerate GmbH, Germany). Anterior edge of the BL was segmented. A Bowman's roughness index (BRI) was defined as the sum of the enclosed areas between segmented edge and a smooth 3(rd) order polynomial fit to the edge. BRI was compared with corneal aberrations, Keratoconus percentage index (KISA), Cone location magnitude index (CLMI) and Belin-Ambrosio enhanced ectasia display overall deviation index (BAD-D). Area under the curve (AUC) was determined with logistic regression (LR). Mean BRI (x10(-3)) was 2.12 mm(2), 1.81 mm(2) and 1.7 mm(2) in normal (n = 26), FFKC and KC eyes, respectively (p <0.001). BAD-D (0.79) and BRI (0.74) had the best AUC for FFKC. By combining BAD-D and BRI, the AUC improved to 0.85 (p = 0.01). For KC eyes, KISA (0.94), CLMI (0.88), BAD-D (0.96) and aberrations had comparable AUC (p > 0.05). However, LR with BRI and other indices didn't improve the AUC in KC eyes (p > 0.05). BRI was significantly reduced in FFKC and KC eyes. It improved the detection of FFKC but not KC eyes.