@article{b6f3cd92fae34569bdac7cfe534b13c1,
title = "Apocryphal FADS2 activity promotes fatty acid diversification in cancer",
abstract = "Canonical fatty acid metabolism describes specific enzyme-substrate interactions that result in products with well-defined chain lengths, degree(s), and positions of unsaturation. Deep profiling of lipids across a range of prostate cancer cell lines reveals a variety of fatty acids with unusual site(s) of unsaturation that are not described by canonical pathways. The structure and abundance of these unusual lipids correlate with changes in desaturase expression and are strong indicators of cellular phenotype. Gene silencing and stable isotope tracing demonstrate that direct Delta 6 and Delta 8 desaturation of 14:0 (myristic), 16:0 (palmitic), and 18:0 (stearic) acids by FADS2 generate new families of unsaturated fatty acids (including n-8, n-10, and n-12) that have rarely-if ever-been reported in human-derived cells. Isomer-resolved lipidomics reveals the selective incorporation of these unusual fatty acids into complex structural lipids and identifies their presence in cancer tissues, indicating functional roles in membrane structure and signaling.",
keywords = "de-novo lipogenesis, delta-6, elongases, elucidation, identification, metabolism, ozone-induced dissociation, position, quantitation, stearoyl-coa desaturase, ELUCIDATION, DE-NOVO LIPOGENESIS, QUANTITATION, IDENTIFICATION, POSITION, STEAROYL-COA DESATURASE, METABOLISM, ELONGASES, DELTA-6, OZONE-INDUCED DISSOCIATION",
author = "R.S.E. Young and A.P. Bowman and E.D. Williams and K.D. Tousignant and C.L. Bidgood and V.R. Narreddula and R. Gupta and D.L. Marshall and B.L.J. Poad and C.C. Nelson and S.R. Ellis and R.M.A. Heeren and M.C. Sadowski and S.J. Blanksby",
note = "Funding Information: MALDI-MSI OzID technology was developed through an industry linkage supported by Waters Corporation and the Australian Research Council (LP180100238). S.J.B. holds patents on ozone-induced dissociation technology (“A method for the determination of the position of unsaturation in a compound,” US8242439 and US7771943). Funding Information: Part of this study was supported by the Movember Foundation, the Prostate Cancer Foundation of Australia and the Australian Government Department of Health through a Movember Revolutionary Team Award, and an Australian Government grant. Other data were obtained at the Central Analytical Research Facility operated by the Institute for Future Environments and supported by funding from the Science and Engineering Faculty (QUT, Brisbane, Australia). This work was supported through funding from the Australian Research Council (DP150101715, LP180100238, and DP190101486) and an Australian Government RTP scholarship. A.P.B. R.M.A.H. and S.R.E. are grateful for funding from Interreg V EMR and the Netherlands Ministry of Economic Affairs within the ?EURLIPIDS? project (EMR23). Conceptualization, R.S.E.Y. M.C.S. S.J.B.; methodology, R.S.E.Y. V.R.N. R.G. D.L.M. B.L.J.P. S.R.E. M.C.S. S.J.B.; software, R.S.E.Y.; validation, R.S.E.Y. A.P.B. K.D.T. V.R.N. M.C.S.; formal analysis, R.S.E.Y. M.C.S.; investigation, R.S.E.Y. A.P.B. E.D.W. K.D.T. C.L.B. M.C.S.; data curation, R.S.E.Y.; writing ? original draft, R.S.E.Y.; writing ? review & editing, R.S.E.Y. E.D.W. D.L.M. B.L.J.P. R.M.A.H. M.C.S. S.J.B.; visualization, R.S.E.Y.; project administration, R.S.E.Y. M.C.S. S.J.B.; resources, E.D.W. B.L.J.P. C.C.N. S.R.E. R.M.A.H. S.J.B.; funding acquisition, B.L.J.P. C.C.N. S.R.E. R.M.A.H. S.J.B.; supervision, M.C.S. S.J.B. MALDI-MSI OzID technology was developed through an industry linkage supported by Waters Corporation and the Australian Research Council (LP180100238). S.J.B. holds patents on ozone-induced dissociation technology (?A method for the determination of the position of unsaturation in a compound,? US8242439 and US7771943). Funding Information: Part of this study was supported by the Movember Foundation , the Prostate Cancer Foundation of Australia and the Australian Government Department of Health through a Movember Revolutionary Team Award , and an Australian Government grant. Other data were obtained at the Central Analytical Research Facility operated by the Institute for Future Environments and supported by funding from the Science and Engineering Faculty (QUT, Brisbane, Australia). This work was supported through funding from the Australian Research Council ( DP150101715 , LP180100238 , and DP190101486 ) and an Australian Government RTP scholarship . A.P.B., R.M.A.H., and S.R.E. are grateful for funding from Interreg V EMR and the Netherlands Ministry of Economic Affairs within the “EURLIPIDS” project (EMR23). Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = feb,
day = "9",
doi = "10.1016/j.celrep.2021.108738",
language = "English",
volume = "34",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "6",
}