Anti-Phosphohistone H3-Positive Mitoses Are Linked to Pathological Response in Neoadjuvantly Treated Breast Cancer

Martin Sillem, Sylvia Timme, Peter Bronsert, Lioudmila Bogatyreva, Dieter Hauschke, Axel zur Hausen, Martin Werner, Elmar Stickeler*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Web of Science)

Abstract

Background: We evaluated breast cancer (BC) core biopsies taken before neoadjuvant chemotherapy (NACT) by immunohistochemistry using anti-phosphohistone H3 (PHH3) antibody to determine mitosis, and correlated the results to clinicopathological data and histopathological regression of resected tumor specimens after NACT. Methods: 72 patients with either triple-negative (TN) or luminal type BC received NACT with epirubicin/cyclo-phosphamide (EC) and Taxotere((R)). Tumor regression was analyzed in resected specimens; pathological complete response (pCR) was achieved in 22.2%. Immunohistochemistry with PHH3 was performed on biopsy samples taken before treatment, and mitotic figures were evaluated in 10 high-power fields (HPF). Results: PHH3-detected mitoses correlated significantly with tumor grading (p = 0.001). TNBC showed > 10 PHH3-positive mitoses/10 HPF significantly more frequently than luminal type BC (p = 0.003). Tumors with > 10 PHH3-positive mitoses/10 HPF achieved pCR significantly more often than those with <10 PHH3-positive mitoses/10 HPF (p = 0.031). Even luminal type BC with > 10 PHH3-positive mitoses/10 HPF was associated significantly with pCR compared to luminal type BC with 10 PHH3-positive mitoses/10 HPF). Immunohistochemical determination of mitoses using anti-PHH3 antibody is a simple and robust method for predicting therapy response to NACT in BC tissue. (C) 2017 S. Karger GmbH, Freiburg

Original languageEnglish
Pages (from-to)244-250
Number of pages7
JournalBreast Care
Volume12
Issue number4
DOIs
Publication statusPublished - 2017

Keywords

  • Breast cancer
  • Mitotic count
  • PHH3
  • Neoadjuvant therapy
  • SURGICAL ADJUVANT BREAST
  • PREOPERATIVE CHEMOTHERAPY
  • PROGNOSTIC VALUE
  • PROLIFERATION
  • CARCINOMA
  • PHOSPHORYLATION
  • RECOMMENDATIONS
  • CONDENSATION
  • EXPRESSION
  • CONSENSUS

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