AnnexinA5-pHrodo: a new molecular probe for measuring efferocytosis

R. Stoehr*, N. Deckers, L. Schurgers, N. Marx, C. P. Reutelingsperger

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Efferocytosis, the clearing of dead or dying cells from living tissues, is a highly programmed, vital process to maintain the healthy functioning of every organism. Disorders of efferocytosis have been linked to several chronic diseases including atherosclerosis and auto-immune diseases. To date several different assays to determine phagocytosis, using microscopy or FACS analysis with labelled targets, have been developed. However, many of these are unable to differentiate between cells that have truly been phagocytosed and those still present on the surface of the macrophages hindering exact assessment of efferocytotic capacity. We herein describe AnxA5-pHrodo and its negative control M1234-pHrodo as new molecular probes to measure in vitro as well as ex-vivo efferocytotic capacity.

Original languageEnglish
Article number17731
Number of pages9
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 7 Dec 2018

Keywords

  • APOPTOTIC CELLS
  • ANNEXIN A5
  • PHOSPHATIDYLSERINE
  • PHAGOCYTOSIS
  • EXPOSURE
  • MACROPHAGES
  • ENGULFMENT
  • CLEARANCE
  • BINDING

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