Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome

Muriel X. G. Draht; Kim M. Smits; Valerie Jooste; Benjamin Tournier; Martijn Vervoort; Chantal Ramaekers; Caroline Chapusot; Matty P. Weijenberg; Manon van Engeland; Veerle Melotte

doi:10.1186/s13148-016-0211-8

Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome

Muriel X. G. Draht, Kim M. Smits, Valerie Jooste, Benjamin Tournier, Martijn Vervoort, Chantal Ramaekers, Caroline Chapusot, Matty P. Weijenberg, Manon van Engeland, Veerle Melotte^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Original language	English
Article number	44
Journal	Clinical epigenetics
Volume	8
DOIs	https://doi.org/10.1186/s13148-016-0211-8
Publication status	Published - 26 Apr 2016

Keywords

DNA methylation
RET
Clinical sensitivity
Analytic sensitivity
MSP
pyrosequencing
MS-HRM

Access to Document

10.1186/s13148-016-0211-8

Cite this

Draht, M. X. G., Smits, K. M., Jooste, V., Tournier, B., Vervoort, M., Ramaekers, C., Chapusot, C., Weijenberg, M. P., van Engeland, M., & Melotte, V. (2016). Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome. Clinical epigenetics, 8, [44]. https://doi.org/10.1186/s13148-016-0211-8

Draht, Muriel X. G. ; Smits, Kim M. ; Jooste, Valerie ; Tournier, Benjamin ; Vervoort, Martijn ; Ramaekers, Chantal ; Chapusot, Caroline ; Weijenberg, Matty P. ; van Engeland, Manon ; Melotte, Veerle. / Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome. In: Clinical epigenetics. 2016 ; Vol. 8.

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title = "Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome",

keywords = "DNA methylation, RET, Clinical sensitivity, Analytic sensitivity, MSP, pyrosequencing, MS-HRM",

author = "Draht, {Muriel X. G.} and Smits, {Kim M.} and Valerie Jooste and Benjamin Tournier and Martijn Vervoort and Chantal Ramaekers and Caroline Chapusot and Weijenberg, {Matty P.} and {van Engeland}, Manon and Veerle Melotte",

year = "2016",

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day = "26",

doi = "10.1186/s13148-016-0211-8",

language = "English",

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Draht, MXG, Smits, KM, Jooste, V, Tournier, B, Vervoort, M, Ramaekers, C, Chapusot, C, Weijenberg, MP , van Engeland, M & Melotte, V 2016, 'Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome', Clinical epigenetics, vol. 8, 44. https://doi.org/10.1186/s13148-016-0211-8

Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome. / Draht, Muriel X. G.; Smits, Kim M.; Jooste, Valerie; Tournier, Benjamin; Vervoort, Martijn; Ramaekers, Chantal; Chapusot, Caroline; Weijenberg, Matty P.; van Engeland, Manon ; Melotte, Veerle.

In: Clinical epigenetics, Vol. 8, 44, 26.04.2016.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome

AU - Draht, Muriel X. G.

AU - Smits, Kim M.

AU - Jooste, Valerie

AU - Tournier, Benjamin

AU - Vervoort, Martijn

AU - Ramaekers, Chantal

AU - Chapusot, Caroline

AU - Weijenberg, Matty P.

AU - van Engeland, Manon

AU - Melotte, Veerle

PY - 2016/4/26

Y1 - 2016/4/26

KW - DNA methylation

KW - RET

KW - Clinical sensitivity

KW - Analytic sensitivity

KW - MSP

KW - pyrosequencing

KW - MS-HRM

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DO - 10.1186/s13148-016-0211-8

M3 - Article

C2 - 27118999

VL - 8

JO - Clinical epigenetics

JF - Clinical epigenetics

SN - 1868-7083

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ER -