Analysis of oxidative DNA damage and HPRT mutant frequencies in cancer patients before and after radiotherapy.

I.H. Zwingmann; I.J. Welle; J.J.M. Engelen; P.A.E.L. Schilderman-Houba; J.M.A. de Jong; J.C.S. Kleinjans

doi:10.1016/S0027-5107(99)00179-7

Analysis of oxidative DNA damage and HPRT mutant frequencies in cancer patients before and after radiotherapy.

I.H. Zwingmann
, I.J. Welle
, J.J.M. Engelen
, P.A.E.L. Schilderman-Houba
, J.M.A. de Jong
, J.C.S. Kleinjans^*

^*Corresponding author for this work

NUTRIM - Institute of Nutrition and Translational Research in Metabolism

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Department of Health Risk Analysis and Toxicology, Maastricht University, Netherlands.

Various markers of radiation-induced DNA damage including DNA oxidation were investigated in peripheral lymphocytes of 23 cancer patients prior to and one week after receiving radiotherapy with a cumulative dose of 54-70 Gy. Exposure to ionizing radiation nonsignificantly increased the ratio 2'deoxy-7-dihydro-8-oxoguanosine/2'deoxyguanosine (8-oxodG/dG) from 1.73 x 10(-5) to 3.33 x 10(-5). Frequencies of micronuclei significantly (p = 0.0003) increased from 6.4 to 38.9 per 1000 cells. The frequency of hypoxanthine-guanine-phosphoribosyltransferase (HPRT) mutant lymphocytes measured as 6-thioguanine resistant variant cells by 5-bromodeoxyuridine labeling, was elevated eight-fold, from 4.7 x 10(-6) to 36.2 x 10(-6) (p = 0.008) after termination of the radiotherapy, thus showing a clear response to the radiation treatment. No correlation between levels of oxidative DNA damage and frequencies of HPRT mutant lymphocytes or micronuclei could be established.

Original language	English
Pages (from-to)	361-369
Number of pages	9
Journal	Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis
Volume	431
Issue number	2
DOIs	https://doi.org/10.1016/S0027-5107(99)00179-7
Publication status	Published - 1 Jan 1999

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Zwingmann, I. H., Welle, I. J., Engelen, J. J. M., Schilderman-Houba, P. A. E. L., de Jong, J. M. A., & Kleinjans, J. C. S. (1999). Analysis of oxidative DNA damage and HPRT mutant frequencies in cancer patients before and after radiotherapy. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 431(2), 361-369. https://doi.org/10.1016/S0027-5107(99)00179-7

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title = "Analysis of oxidative DNA damage and HPRT mutant frequencies in cancer patients before and after radiotherapy.",

abstract = "Department of Health Risk Analysis and Toxicology, Maastricht University, Netherlands. Various markers of radiation-induced DNA damage including DNA oxidation were investigated in peripheral lymphocytes of 23 cancer patients prior to and one week after receiving radiotherapy with a cumulative dose of 54-70 Gy. Exposure to ionizing radiation nonsignificantly increased the ratio 2'deoxy-7-dihydro-8-oxoguanosine/2'deoxyguanosine (8-oxodG/dG) from 1.73 x 10(-5) to 3.33 x 10(-5). Frequencies of micronuclei significantly (p = 0.0003) increased from 6.4 to 38.9 per 1000 cells. The frequency of hypoxanthine-guanine-phosphoribosyltransferase (HPRT) mutant lymphocytes measured as 6-thioguanine resistant variant cells by 5-bromodeoxyuridine labeling, was elevated eight-fold, from 4.7 x 10(-6) to 36.2 x 10(-6) (p = 0.008) after termination of the radiotherapy, thus showing a clear response to the radiation treatment. No correlation between levels of oxidative DNA damage and frequencies of HPRT mutant lymphocytes or micronuclei could be established.",

author = "I.H. Zwingmann and I.J. Welle and J.J.M. Engelen and P.A.E.L. Schilderman-Houba and \{de Jong\}, J.M.A. and J.C.S. Kleinjans",

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doi = "10.1016/S0027-5107(99)00179-7",

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AU - Zwingmann, I.H.

AU - Welle, I.J.

AU - Engelen, J.J.M.

AU - Schilderman-Houba, P.A.E.L.

AU - de Jong, J.M.A.

AU - Kleinjans, J.C.S.

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N2 - Department of Health Risk Analysis and Toxicology, Maastricht University, Netherlands. Various markers of radiation-induced DNA damage including DNA oxidation were investigated in peripheral lymphocytes of 23 cancer patients prior to and one week after receiving radiotherapy with a cumulative dose of 54-70 Gy. Exposure to ionizing radiation nonsignificantly increased the ratio 2'deoxy-7-dihydro-8-oxoguanosine/2'deoxyguanosine (8-oxodG/dG) from 1.73 x 10(-5) to 3.33 x 10(-5). Frequencies of micronuclei significantly (p = 0.0003) increased from 6.4 to 38.9 per 1000 cells. The frequency of hypoxanthine-guanine-phosphoribosyltransferase (HPRT) mutant lymphocytes measured as 6-thioguanine resistant variant cells by 5-bromodeoxyuridine labeling, was elevated eight-fold, from 4.7 x 10(-6) to 36.2 x 10(-6) (p = 0.008) after termination of the radiotherapy, thus showing a clear response to the radiation treatment. No correlation between levels of oxidative DNA damage and frequencies of HPRT mutant lymphocytes or micronuclei could be established.

AB - Department of Health Risk Analysis and Toxicology, Maastricht University, Netherlands. Various markers of radiation-induced DNA damage including DNA oxidation were investigated in peripheral lymphocytes of 23 cancer patients prior to and one week after receiving radiotherapy with a cumulative dose of 54-70 Gy. Exposure to ionizing radiation nonsignificantly increased the ratio 2'deoxy-7-dihydro-8-oxoguanosine/2'deoxyguanosine (8-oxodG/dG) from 1.73 x 10(-5) to 3.33 x 10(-5). Frequencies of micronuclei significantly (p = 0.0003) increased from 6.4 to 38.9 per 1000 cells. The frequency of hypoxanthine-guanine-phosphoribosyltransferase (HPRT) mutant lymphocytes measured as 6-thioguanine resistant variant cells by 5-bromodeoxyuridine labeling, was elevated eight-fold, from 4.7 x 10(-6) to 36.2 x 10(-6) (p = 0.008) after termination of the radiotherapy, thus showing a clear response to the radiation treatment. No correlation between levels of oxidative DNA damage and frequencies of HPRT mutant lymphocytes or micronuclei could be established.

U2 - 10.1016/S0027-5107(99)00179-7

DO - 10.1016/S0027-5107(99)00179-7

M3 - Article

SN - 0027-5107

VL - 431

SP - 361

EP - 369

JO - Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis

IS - 2

ER -

Analysis of oxidative DNA damage and HPRT mutant frequencies in cancer patients before and after radiotherapy.

Abstract

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