Analyses of human colonic mucus obtained by an in vivo sampling technique.

H.M.H. Hamer; D.M. Jonkers; A. Loof; S.A. Vanhoutvin; F.J. Troost; K. Venema; A. Kodde; G.H. Koek; R.G. Schipper; W.L. van Heerde; R.J.M. Brummer

doi:10.1016/j.dld.2008.12.100

Analyses of human colonic mucus obtained by an in vivo sampling technique.

H.M.H. Hamer^*, D.M. Jonkers, A. Loof, S.A. Vanhoutvin, F.J. Troost, K. Venema, A. Kodde, G.H. Koek, R.G. Schipper, W.L. van Heerde, R.J.M. Brummer

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: The mucus layer is an important dynamic component of the epithelial barrier. It contains mucin glycoproteins and other compounds secreted by the intestinal epithelium, such as secretory IgA. However, a standardized in vivo sampling technique of mucus in humans is not yet available. AIM: To assess the validity and feasibility of mucin and protein determinations in human colonic mucus collected under physiological conditions. SUBJECTS AND METHODS: Triplicate colonic mucus samples were collected in 11 healthy volunteers using cytology brushes during sigmoidoscopy. As an indication of the quantity of collected mucus, total protein and mucin concentrations were determined by measuring oligosaccharide equivalents and monosaccharides. Also secretory IgA and sialic acid concentrations were determined and proteomic analysis was performed using surface enhanced laser desorption/ionization-time of flight-mass spectrometry. RESULTS: Mean values of secretory IgA and sialic acid corrected for the amount of mucus ranged from 0.16 to 1.81g secretory IgA/mmol oligosaccharide equivalents and from 12.6 to 48.6g sialic acid/mmol oligosaccharide equivalents. Proteomic analysis of mucus is feasible and cluster analysis showed subject specific profiles. CONCLUSION: Using cytology brushes, human colonic mucus can be sampled and under physiological conditions. These samples could give information on the composition and quality of the mucus layer.

Original language	English
Pages (from-to)	559-564
Journal	Digestive and Liver Disease
Volume	41
Issue number	8
DOIs	https://doi.org/10.1016/j.dld.2008.12.100
Publication status	Published - 1 Jan 2009

Access to Document

10.1016/j.dld.2008.12.100

Cite this

@article{ed1113af1a594d34a2b2d20c9afa61e0,

title = "Analyses of human colonic mucus obtained by an in vivo sampling technique.",

abstract = "BACKGROUND: The mucus layer is an important dynamic component of the epithelial barrier. It contains mucin glycoproteins and other compounds secreted by the intestinal epithelium, such as secretory IgA. However, a standardized in vivo sampling technique of mucus in humans is not yet available. AIM: To assess the validity and feasibility of mucin and protein determinations in human colonic mucus collected under physiological conditions. SUBJECTS AND METHODS: Triplicate colonic mucus samples were collected in 11 healthy volunteers using cytology brushes during sigmoidoscopy. As an indication of the quantity of collected mucus, total protein and mucin concentrations were determined by measuring oligosaccharide equivalents and monosaccharides. Also secretory IgA and sialic acid concentrations were determined and proteomic analysis was performed using surface enhanced laser desorption/ionization-time of flight-mass spectrometry. RESULTS: Mean values of secretory IgA and sialic acid corrected for the amount of mucus ranged from 0.16 to 1.81g secretory IgA/mmol oligosaccharide equivalents and from 12.6 to 48.6g sialic acid/mmol oligosaccharide equivalents. Proteomic analysis of mucus is feasible and cluster analysis showed subject specific profiles. CONCLUSION: Using cytology brushes, human colonic mucus can be sampled and under physiological conditions. These samples could give information on the composition and quality of the mucus layer.",

author = "H.M.H. Hamer and D.M. Jonkers and A. Loof and S.A. Vanhoutvin and F.J. Troost and K. Venema and A. Kodde and G.H. Koek and R.G. Schipper and {van Heerde}, W.L. and R.J.M. Brummer",

year = "2009",

month = jan,

day = "1",

doi = "10.1016/j.dld.2008.12.100",

language = "English",

volume = "41",

pages = "559--564",

journal = "Digestive and Liver Disease",

issn = "1590-8658",

publisher = "Elsevier Science",

number = "8",

}

TY - JOUR

T1 - Analyses of human colonic mucus obtained by an in vivo sampling technique.

AU - Hamer, H.M.H.

AU - Jonkers, D.M.

AU - Loof, A.

AU - Vanhoutvin, S.A.

AU - Troost, F.J.

AU - Venema, K.

AU - Kodde, A.

AU - Koek, G.H.

AU - Schipper, R.G.

AU - van Heerde, W.L.

AU - Brummer, R.J.M.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - BACKGROUND: The mucus layer is an important dynamic component of the epithelial barrier. It contains mucin glycoproteins and other compounds secreted by the intestinal epithelium, such as secretory IgA. However, a standardized in vivo sampling technique of mucus in humans is not yet available. AIM: To assess the validity and feasibility of mucin and protein determinations in human colonic mucus collected under physiological conditions. SUBJECTS AND METHODS: Triplicate colonic mucus samples were collected in 11 healthy volunteers using cytology brushes during sigmoidoscopy. As an indication of the quantity of collected mucus, total protein and mucin concentrations were determined by measuring oligosaccharide equivalents and monosaccharides. Also secretory IgA and sialic acid concentrations were determined and proteomic analysis was performed using surface enhanced laser desorption/ionization-time of flight-mass spectrometry. RESULTS: Mean values of secretory IgA and sialic acid corrected for the amount of mucus ranged from 0.16 to 1.81g secretory IgA/mmol oligosaccharide equivalents and from 12.6 to 48.6g sialic acid/mmol oligosaccharide equivalents. Proteomic analysis of mucus is feasible and cluster analysis showed subject specific profiles. CONCLUSION: Using cytology brushes, human colonic mucus can be sampled and under physiological conditions. These samples could give information on the composition and quality of the mucus layer.

AB - BACKGROUND: The mucus layer is an important dynamic component of the epithelial barrier. It contains mucin glycoproteins and other compounds secreted by the intestinal epithelium, such as secretory IgA. However, a standardized in vivo sampling technique of mucus in humans is not yet available. AIM: To assess the validity and feasibility of mucin and protein determinations in human colonic mucus collected under physiological conditions. SUBJECTS AND METHODS: Triplicate colonic mucus samples were collected in 11 healthy volunteers using cytology brushes during sigmoidoscopy. As an indication of the quantity of collected mucus, total protein and mucin concentrations were determined by measuring oligosaccharide equivalents and monosaccharides. Also secretory IgA and sialic acid concentrations were determined and proteomic analysis was performed using surface enhanced laser desorption/ionization-time of flight-mass spectrometry. RESULTS: Mean values of secretory IgA and sialic acid corrected for the amount of mucus ranged from 0.16 to 1.81g secretory IgA/mmol oligosaccharide equivalents and from 12.6 to 48.6g sialic acid/mmol oligosaccharide equivalents. Proteomic analysis of mucus is feasible and cluster analysis showed subject specific profiles. CONCLUSION: Using cytology brushes, human colonic mucus can be sampled and under physiological conditions. These samples could give information on the composition and quality of the mucus layer.

U2 - 10.1016/j.dld.2008.12.100

DO - 10.1016/j.dld.2008.12.100

M3 - Article

C2 - 19213618

SN - 1590-8658

VL - 41

SP - 559

EP - 564

JO - Digestive and Liver Disease

JF - Digestive and Liver Disease

IS - 8

ER -