TY - JOUR
T1 - An in vitro and in vivo study of peptide-functionalized nanoparticles for brain targeting
T2 - The importance of selective blood-brain barrier uptake
AU - Bode, Gerard H.
AU - Coue, Gregory
AU - Freese, Christian
AU - Pickl, Karin E.
AU - Sanchez-Purra, Maria
AU - Albaiges, Berta
AU - Borros, Salvador
AU - van Winden, Ewoud C.
AU - Tziveleka, Leto-Aikaterini
AU - Sideratou, Zili
AU - Engbersen, Johan F. J.
AU - Singh, Smriti
AU - Albrecht, Krystyna
AU - Groll, Juergen
AU - Moeller, Martin
AU - Poetgens, Andy J. G.
AU - Schmitzk, Christoph
AU - Froehlich, Eleonore
AU - Grandfils, Christian
AU - Sinner, Frank M.
AU - Kirkpatrick, C. James
AU - Steinbusch, Harry W. M.
AU - Frank, Hans-Georg
AU - Unger, Ronald E.
AU - Martinez-Martinez, Pilar
PY - 2017/4
Y1 - 2017/4
N2 - Targeted delivery of drugs across endothelial barriers remains a formidable challenge, especially in the case of the brain, where the blood-brain barrier severely limits entry of drugs into the central nervous system. Nanoparticle-mediated transport of peptide/protein-based drugs across endothelial barriers shows great potential as a therapeutic strategy in a wide variety of diseases. Functionalizing nanoparticles with peptides allows for more efficient targeting to specific organs. We have evaluated the hemocompatibilty, cytotoxicity, endothelial uptake, efficacy of delivery and safety of liposome, hyperbranched polyester, poly(glycidol) and acrylamide-based nanoparticles functionalized with peptides targeting brain endothelial receptors, in vitro and in vivo. We used an ELISA-based method for the detection of nanoparticles in biological fluids, investigating the blood clearance rate and in vivo biodistribution of labeled nanoparticles in the brain after intravenous injection in Wistar rats. Herein, we provide a detailed report of in vitro and in vivo observations. (C) 2016 Elsevier Inc. All rights reserved.
AB - Targeted delivery of drugs across endothelial barriers remains a formidable challenge, especially in the case of the brain, where the blood-brain barrier severely limits entry of drugs into the central nervous system. Nanoparticle-mediated transport of peptide/protein-based drugs across endothelial barriers shows great potential as a therapeutic strategy in a wide variety of diseases. Functionalizing nanoparticles with peptides allows for more efficient targeting to specific organs. We have evaluated the hemocompatibilty, cytotoxicity, endothelial uptake, efficacy of delivery and safety of liposome, hyperbranched polyester, poly(glycidol) and acrylamide-based nanoparticles functionalized with peptides targeting brain endothelial receptors, in vitro and in vivo. We used an ELISA-based method for the detection of nanoparticles in biological fluids, investigating the blood clearance rate and in vivo biodistribution of labeled nanoparticles in the brain after intravenous injection in Wistar rats. Herein, we provide a detailed report of in vitro and in vivo observations. (C) 2016 Elsevier Inc. All rights reserved.
KW - Nanoparticles
KW - Peptides
KW - Brain
KW - Targeting
KW - 80-COATED POLYBUTYLCYANOACRYLATE NANOPARTICLES
KW - DRUG-DELIVERY
KW - SENSITIVE HYDROGELS
KW - MEDIATED TRANSPORT
KW - DENDRITIC POLYMERS
KW - NANOGELS
KW - CELLS
KW - DENDRIMERS
KW - LIPOSOMES
KW - METHOTREXATE
U2 - 10.1016/j.nano.2016.11.009
DO - 10.1016/j.nano.2016.11.009
M3 - Article
C2 - 27884636
SN - 1549-9634
VL - 13
SP - 1289
EP - 1300
JO - Nanomedicine-Nanotechnology Biology and Medicine
JF - Nanomedicine-Nanotechnology Biology and Medicine
IS - 3
ER -