An efficient protocol towards site-specifically clickable nanobodies in high yield: cytoplasmic expression in Escherichia coli combined with intein-mediated protein ligation

Duy Tien Ta, Erik Steen Redeker, Brecht Billen, Gunter Reekmans, Josephine Sikulu, Jean-Paul Noben, Wanda Guedens, Peter Adriaensens*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Original languageEnglish
Pages (from-to)351-363
Number of pages13
JournalProtein Engineering Design & Selection
Volume28
Issue number10
DOIs
Publication statusPublished - Oct 2015

Keywords

  • bioorthogonal chemistry
  • CuAAC
  • cytoplasmic expression
  • intein-mediated protein ligation
  • VCAM1-targeting nanobody
  • SINGLE-DOMAIN ANTIBODIES
  • CELL-ADHESION MOLECULE-1
  • RECOMBINANT EXPRESSION
  • CHEMICAL LIGATION
  • PURIFICATION
  • CANCER
  • IMMOBILIZATION
  • FRAGMENTS
  • IMMUNOGLOBULINS
  • BIOCONJUGATION

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