TY - JOUR
T1 - Amygdalar nuclei and hippocampal subfields on MRI
T2 - Test-retest reliability of automated volumetry across different MRI sites and vendors
AU - Quattrini, Giulia
AU - Pievani, Michela
AU - Jovicich, Jorge
AU - Aiello, Marco
AU - Bargallo, Nuria
AU - Barkhof, Frederik
AU - Bartres-Faz, David
AU - Beltramello, Alberto
AU - Pizzini, Francesca B.
AU - Blin, Olivier
AU - Bordet, Regis
AU - Caulo, Massimo
AU - Constantinides, Manos
AU - Didic, Mira
AU - Drevelegas, Antonios
AU - Ferretti, Antonio
AU - Fiedler, Ute
AU - Floridi, Piero
AU - Gros-Dagnac, Helene
AU - Hoffmann, Karl-Titus
AU - Hensch, Tilman
AU - Kuijer, Joost P.
AU - Lopes, Renaud
AU - Marra, Camillo
AU - Mueller, Bernhard W.
AU - Nobili, Flavio
AU - Parnetti, Lucilla
AU - Payoux, Pierre
AU - Picco, Agnese
AU - Ranjeva, Jean-Philippe
AU - Roccatagliata, Luca
AU - Rossini, Paolo M.
AU - Salvatore, Marco
AU - Schonknecht, Peter
AU - Schott, Bjoern H.
AU - Sein, Julien
AU - Soricelli, Andrea
AU - Tarducci, Roberto
AU - Tsolaki, Magda
AU - Visser, Pieter J.
AU - Wiltfang, Jens
AU - Richardson, Jill C.
AU - Frisoni, Giovanni B.
AU - Marizzoni, Moira
AU - PharmaCog Consortium
PY - 2020/9
Y1 - 2020/9
N2 - Background: The amygdala and the hippocampus are two limbic structures that play a critical role in cognition and behavior, however their manual segmentation and that of their smaller nuclei/subfields in multicenter datasets is time consuming and difficult due to the low contrast of standard MRI. Here, we assessed the reliability of the automated segmentation of amygdalar nuclei and hippocampal subfields across sites and vendors using FreeSurfer in two independent cohorts of older and younger healthy adults.Methods: Sixty-five healthy older (cohort 1) and 68 younger subjects (cohort 2), from the PharmaCog and CoRR consortia, underwent repeated 3D-T1 MRI (interval 1-90 days). Segmentation was performed using FreeSurfer v6.0. Reliability was assessed using volume reproducibility error (epsilon) and spatial overlapping coefficient (DICE) between test and retest session.Results: Significant MRI site and vendor effects (p <.05) were found in a few subfields/nuclei for the epsilon, while extensive effects were found for the DICE score of most subfields/nuclei. Reliability was strongly influenced by volume, as e correlated negatively and DICE correlated positively with volume size of structures (absolute value of Spearman's r correlations >0.43, p <1.39E-36). In particular, volumes larger than 200 mm(3) (for amygdalar nuclei) and 300 mm(3) (for hippocampal subfields, except for molecular layer) had the best test-retest reproducibility (epsilon <5% and DICE > 0.80).Conclusion: Our results support the use of volumetric measures of larger amygdalar nuclei and hippocampal subfields in multisite MRI studies. These measures could be useful for disease tracking and assessment of efficacy in drug trials.
AB - Background: The amygdala and the hippocampus are two limbic structures that play a critical role in cognition and behavior, however their manual segmentation and that of their smaller nuclei/subfields in multicenter datasets is time consuming and difficult due to the low contrast of standard MRI. Here, we assessed the reliability of the automated segmentation of amygdalar nuclei and hippocampal subfields across sites and vendors using FreeSurfer in two independent cohorts of older and younger healthy adults.Methods: Sixty-five healthy older (cohort 1) and 68 younger subjects (cohort 2), from the PharmaCog and CoRR consortia, underwent repeated 3D-T1 MRI (interval 1-90 days). Segmentation was performed using FreeSurfer v6.0. Reliability was assessed using volume reproducibility error (epsilon) and spatial overlapping coefficient (DICE) between test and retest session.Results: Significant MRI site and vendor effects (p <.05) were found in a few subfields/nuclei for the epsilon, while extensive effects were found for the DICE score of most subfields/nuclei. Reliability was strongly influenced by volume, as e correlated negatively and DICE correlated positively with volume size of structures (absolute value of Spearman's r correlations >0.43, p <1.39E-36). In particular, volumes larger than 200 mm(3) (for amygdalar nuclei) and 300 mm(3) (for hippocampal subfields, except for molecular layer) had the best test-retest reproducibility (epsilon <5% and DICE > 0.80).Conclusion: Our results support the use of volumetric measures of larger amygdalar nuclei and hippocampal subfields in multisite MRI studies. These measures could be useful for disease tracking and assessment of efficacy in drug trials.
KW - Amygdalar nuclei
KW - Hippocampal subfields
KW - Multicenter MRI study
KW - FreeSurfer
KW - Reliability analysis
KW - BORDERLINE PERSONALITY-DISORDER
KW - SAMPLE-SIZE CALCULATIONS
KW - SURFACE-BASED ANALYSIS
KW - ALZHEIMERS-DISEASE
KW - FUNCTIONAL-ORGANIZATION
KW - STRUCTURAL DIFFERENCES
KW - MANUAL SEGMENTATION
KW - METHODS FREESURFER
KW - ELDERLY-PATIENTS
KW - HUMAN BRAIN
U2 - 10.1016/j.neuroimage.2020.116932
DO - 10.1016/j.neuroimage.2020.116932
M3 - Article
C2 - 32416226
SN - 1053-8119
VL - 218
JO - Neuroimage
JF - Neuroimage
M1 - 116932
ER -