Aminooxy-Acetic Acid Inhibits Experimental Autoimmune Uveitis by Modulating the Balance between Effector and Regulatory Lymphocyte Subsets

Suyin Mei, Yike Huang, Na Li, Zongren Xu, Jing Xu, Qinjin Dai, Juan Wu, Aize Kijlstra, Peizeng Yang, Shengping Hou*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Purpose: A small molecular compound, aminooxy-acetic acid (AOA), has been shown to modulate experimental autoimmune encephalomyelitis (EAE). The current study was designed to investigate whether AOA has a similar effect on the development of experimental autoimmune uveitis (EAU) and to further explore underlying mechanisms of this drug.

Methods: EAU was induced in C57BL/6J mice by immunization with interphotoreceptor retinoid-binding protein peptide 651-670 (IRBP 651-670). AOA (500pg or 750 mu g) or vehicle was administered by intraperitoneal injection from day 10 to 14 after EAU induction. The severity was assessed by clinical and histological scores. The integrity of the blood retinal barrier was detected with Evans Blue. Frequencies of splenic Th1, Th17 and Foxp3(+) Treg cells were examined by flow cytometry. The production of cytokines was tested by ELISA. The mRNA expression of IL-17, IFN-gamma and IL-10 was detected by RT-PCR. The expression of p-Stat1 and NF-kappa B was detected by Western Blotting.

Results: AOA was found to markedly inhibit the severity of EAU, as determined by clinical and histopathological examinations. AOA can relieve the leakage of blood retinal barrier (BRB). Functional studies found a decreased frequency of Th1 and Th17 cells and an increased frequency of Treg cells in EAU mice as compared with controls. Further studies showed that AOA not only downregulated the production of the proinflammatory cytokines including IFN-gamma and IL-17 but also upregulated the expression of an anti-inflammatory cytokine such as IL-10, which might be caused by inhibiting the expressions of p-Stat1 and NF-kappa B.

Conclusion: This study shows that AOA inhibits the severity and development of EAU by modulating the balance between regulatory and pathogenic lymphocyte subsets.

Original languageEnglish
Pages (from-to)624-632
Number of pages9
JournalCurrent Molecular Medicine
Issue number8
Publication statusPublished - 2020


  • Experimental autoimmune uveitis
  • aminooxy-acetic acid (AOA)
  • Th17 cell
  • Th1 cell
  • Treg cell
  • Uveitis
  • TH17


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